CO_2浓度和抗药性对褐飞虱刺吸取食行为的影响  

Effects of CO_2 Concentration and Pesticide Resistance on Penetration Behaviors in Nilaparvata lugens(Homoptera:Delphacidae)

作　　者：刘敬[1] 吴珊珊[1] 孟玲[1] 李保平[1] 

机构地区：[1]南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室,南京210095

出　　处：《中国农业科学》2013年第21期4471-4477,共7页Scientia Agricultura Sinica

基　　金：国家公益性行业(农业)科研专项(200903003;201103002);国家科技支撑计划项目(2012BAC19B01)

摘　　要：【目的】明确CO2浓度升高是否影响褐飞虱的取食行为以及抗药褐飞虱品系的刺吸行为是否与敏感品系存在差异,从而为预测CO2浓度升高条件下不同抗药性褐飞虱对水稻的致害性提供依据。【方法】采用刺吸电位技术(EPG)观察褐飞虱抗、感噻嗪酮品系(抗性水平相差约480倍)对当前(390μL·L-1)和升高CO2浓度(780μL·L-1)下生长水稻的口针刺吸行为,用双因素方差分析比较CO2浓度和抗药性2因素对口针刺吸显示的6种波形持续时间和频次的差异。【结果】CO2浓度加倍倾向于缩短感噻嗪酮褐飞虱在韧皮部摄取汁液的总时间,与抗药性互作显著影响开始刺探行为的频次:CO2浓度加倍条件下(780μL·L-1)抗性褐飞虱开始刺探的频次低于当前CO2浓度下(390μL·L-1)。抗噻嗪酮褐飞虱口针朝维管束移动和在木质部摄取汁液的时间延长,但在韧皮部内部活动的时间缩短;口针在韧皮部内部活动的频次增多,但在韧皮部和木质部摄取汁液的频次减少。【结论】抗噻嗪酮褐飞虱对水稻的致害性明显强于感噻嗪酮褐飞虱;CO2浓度升高可能使感噻嗪酮褐飞虱对水稻的致害性减弱,但对抗噻嗪酮褐飞虱没有显著影响。[Objective] This study was designed to clarify if the CO2 enrichment influences the feeding behaviors of Nilaparvata lugens and if there are differences in feeding behaviors between the planthopper varied in pesticide-resistance, which would provide data for an assessment of the virulence to rice plant of pesticide resistant N. lugens under the climate change. [Method] Using the electrical penetration graph （EPG） technique stylet penetration behaviors were recorded to evaluate the performances of buprofezin-susceptible and -resistant strains （a 480-fold difference in resistance） on rice leaves under either ambient （390 pL.L1） or elevated CO2 concentration （780 laL＇LI）. Two-way ANOVAs were used to test the effects of CO2 and pesticide resistance on the six waveforms associated with the plant penetration activities in the total time and frequency. [Result] N. lugens exhibited a tendency to shorten the phloem sap ingestion duration under the CO2 elevation, and the buprofezin-resistant N. lugens decreased the frequency of the penetration initiation under the elevated CO2 concentration （780 gL＇L1） as opposed to the ambient CO2 （390 pL-LI）. In comparison with the buprofezin-susceptible insect, the buprofezin-resistant spent more time for the salvation plus penetration movement and the stylet activity in xylem region, increased the frequency of the intracellular activity in phloem region, but decreased the frequency of the phloem sap ingestion and the stylet activity in xylem region. [Conclusion] The buprofezin-resistant N. lugens can be stronger in virulence to rice than the buprofezin-susceptible. The CO2 enrichment may slightlyminimize the virulence of the buprofezin-susceptible N. lugens but have no obvious effects on the virulence of the buprofezin-resistant N. lugens.

关 键 词：CO2浓度升高 抗药性 刺吸电位技术(EPG) 气候变化 

分 类 号：S433[农业科学—农业昆虫与害虫防治]