湖羊TGF-β1基因特征、表达及其与排卵数的相关性分析  

Characterization,Expression of TGF-β1 Gene and Its Association with Ovulation Rate in Hu Sheep

作　　者：郭晶[1] 李新宇[1] 李隐侠[2] 潘增祥[1] 决肯[3] 刘吉英[1] 李二林[1] 谢庄[1] 陈玲 李齐发[1] 

机构地区：[1]南京农业大学动物科技学院,南京210095 [2]江苏省农业科学院畜牧研究所,南京210014 [3]新疆农业大学动物科学学院,乌鲁木齐830052 [4]江苏省苏州种羊场,江苏苏州215000

出　　处：《中国农业科学》2013年第21期4586-4593,共8页Scientia Agricultura Sinica

基　　金：国家自然科学基金(30671503);江苏省自然科学基金(BK2007156);国家重点基础研究发展计划(2007CB947403)

摘　　要：【目的】获取湖羊TGF-β1基因序列,了解TGF-β1序列特征和组织表达特征,分析卵巢组织中TGF-β1基因表达水平与排卵数之间的关系。【方法】通过PCR扩增和测序获得湖羊TGF-β1基因序列,并采用生物信息学方法分析其序列特征;采用RT-PCR分析湖羊TGF-β1基因的组织表达特征;采用Real-time qPCR方法分析多羔组与单羔组湖羊卵巢组织TGF-β1基因表达水平的差异,及其与排卵数之间的关系。【结果】湖羊TGF-β1基因编码区序列全长1 173 bp,编码蛋白含有390个氨基酸残基,包含典型的TGF-β前肽和TGF-βlike等结构域。TGF-β1基因在湖羊卵巢组织中高表达,多羔组卵巢组织中TGF-β1基因表达水平显著高于单羔组(P<0.05),且表达水平与排卵数呈极显著正相关(P<0.01)。【结论】多羔组湖羊卵巢组织TGF-β1基因表达水平显著高于单羔组,且与排卵数间呈极显著正相关,推测TGF-β1基因在湖羊卵泡发育、成熟或排卵过程中发挥重要作用,是影响湖羊高繁殖力的候选基因。[Objective] The present study was performed to clone the TGF-β1 sequence in Hu sheep and to comprehend the sequence character of Hu sheep so as to investigate the relationships between the mRNA level of TGF-β1 gene and ovulation number in ovaries of Hu sheep. [Method] The sequence of TGF-β1 in Hu sheep was cloned and sequenced, and the sequence was analyzed by bioinformatics. The tissue distributions of TGF-β1 were detected by RT-PCR, and the gene expression level differences in the ovaries between the high- and the low-fecundity groups and the relationships with the ovulation rate were detected by real-time qPCR. [Result] The length ofTGF-β1 gene coding sequence was 1 173 bp, encoding 390 amino acids which include some typical domains such as TGF-β propeptide and TGF-β like. RT-PCR assays revealed that TGF-β1 highly expressed in the ovary tissue. The ovarian mRNA expression level of TGF-β1 in high-fecundity group was significantly greater than that in low-fecundity group （P〈 0.05）, and mRNA expression level was significantly positively correlated with ovulation rate （P〈0.01）. [ Conclusion] The ovarian mRNA expression level of TGF-β1 in high-fecundity group was significantly greater than that in low-fecundity group and significantly positively correlated with ovulation rate. These findings indicate that TGF-β1 of Hu sheep play an important role in follicular development, maturation or ovulation, which probably is associated with high fecundity of Hu sheep.

关 键 词：湖羊 TGF—β1基因 产羔数 排卵数 

分 类 号：S826[农业科学—畜牧学]