大孔树脂纯化显齿蛇葡萄黄酮的工艺研究  

Purification of total flavones from Ampelopsis grossedentata with macroporous resins

作　　者：孔庆童 付明[1,2,3] 胡兴 王登宇[1,2,3] 

机构地区：[1]民族药用植物资源研究与利用湖南省重点实验室 [2]湘西药用植物与民族植物学湖南省高校重点实验室 [3]怀化学院生命科学系,湖南怀化418008

出　　处：《中国民族医药杂志》2013年第9期60-63,共4页Journal of Medicine and Pharmacy of Chinese Minorities

基　　金：湖南省高校科技创新团队支持计划资助(2010212);湖南省"十二五"植物学重点建设学科资助(201142)

摘　　要：采用分光光度法测黄酮的含量,筛选大孔树脂纯化显齿蛇葡萄黄酮的最佳条件。实验结果表明:HPD100树脂的吸附效果最好,当黄酮浓度为137.8 ug/mL、上柱体积为5 mL、吸附30 min,用95%乙醇、以1.5 mL/min流速洗脱时,吸附率为85.44%,洗脱率为94.92%,回收率为95.66%,样品的黄酮质量分数从2.76%纯化至81.1%。实验证明本工艺合理、可行,适合工业化生产。The content of flavonoids was determined by spectrophotometry. Screening the optimum conditions of purification flavonoids from Ampelopsis grossedentata by macroporous resin . The result showed： HPD100 resin is the best for the purification of flavones, when the concentration of crude flavones was 137.8μg . mL- 1 , the volume of sample was 5 mL, the time of adsorption was 30 min, flow rate was 1.5 mL/min and the eluent was 95% ethanol, the adsorption rate was 85.44% , the elution rate of flavones was 94. 92% ,the rate of recovery was 95.66%, Under the optimum conditions the falconoid compounds quality score was from 2.76% to 81.10%. The experiment showed that this technology was reasonable and practicable, suitable for industrial production.

关 键 词：显齿蛇葡萄 黄酮 树脂 纯化 

分 类 号：R297.2[医药卫生—民族医学]