嗜酸乳杆菌对斑马鱼子代幼鱼免疫功能的影响  

Effect of L.acidophilus JCM1132 on Immune Function of Zebrafish (Danio rerio) Offspring

作　　者：张羽[1] 盛洁[1] 李青[2] 余强[2] 邓扬[2] 卿素珠[1] 何夙旭[2] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]中国农业科学院饲料研究所,北京100081

出　　处：《动物医学进展》2013年第11期77-80,共4页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31272672)

摘　　要：以嗜酸乳酸杆菌JCM 1132作为益生菌添加剂饲喂亲代斑马鱼,研究其对子代幼鱼免疫功能的影响。试验设对照组(饲喂基础饲料)和益生菌组(基础饲料中添加108 cfu/g嗜酸乳酸杆菌JCM 1132),饲喂30d后,对其孵化的幼鱼进行嗜水气单胞菌攻毒,分别检测攻毒后幼鱼死亡情况及攻毒期间鱼体内免疫因子il1b和nfkbiaa mRNA相对表达量。结果表明,在攻毒后5d到8d,益生菌组的幼鱼死亡率显著高于对照组(P<0.05);在攻毒后5h、24h和48h,益生菌组幼鱼的il1b和nfkbiaa转录显著低于对照组(P<0.05)。表明嗜酸乳酸杆菌饲喂斑马鱼亲鱼并不能提高子代斑马鱼的免疫功能。The aim of this research is to evalute the effect of dietary L .acidophilus JCM 1132 on the off-spring immunity in zebrafish （Danio rerio） .Sexually-mature male and female zebrafish as brood fish were divided into two groups with different feeding ：basal diets （CTRL） ,basal diets plus 108 cfu/g food L .aci-dophilus JCM 1132 （LA） ,for 30 days .The larvae hatched from two groups were challenged with Aero-monas hydrophila NJ-1 ,and then the mortality and the expression of immune factor il1b and nfkbiaa of larvae were oberved .The results showed that from 5 days to 8 days after challenge ,the mortality of the larva from LA group was higher significantly than that of CK group （P〈0 .05）;at 5 ,24 ,48 hours after challenge ,the expressions of il1b and nfkbiaa in larvae from LA group decreased significantly than that from CK group .This indicated that larval immuinty was not enhenced by feeding their parants with L .ac-idophilus JCM 1132 .

关 键 词：益生菌 嗜酸乳酸杆菌 斑马鱼 子代 免疫 

分 类 号：S852.651[农业科学—基础兽医学] Q786[农业科学—兽医学]