生物絮团中异养亚硝化菌的分离鉴定及其特性  

Isolation and identification of heterotrophic nitrosobacteria from bio-flocs and analysis of their characteristics

作　　者：胡修贵[1,2] 赵培[2] 李玉宏[2,3] 宋晓玲[2] 马甡[1] 黄倢[2] 

机构地区：[1]中国海洋大学水产学院,青岛266003 [2]农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所,青岛266071 [3]上海海洋大学,201306

出　　处：《渔业科学进展》2013年第5期97-103,共7页Progress in Fishery Sciences

基　　金：公益性行业(农业)科研专项经费项目(201103034);现代农业产业技术体系(CARS-47)共同资助

摘　　要：采用富集培养、分离纯化等微生物学手段,从对虾养殖池的生物絮团中筛选出两株对氨氮具有高转化率的菌株.16S rRNA测序及系统发育分析结果表明,两株菌均属于盐单胞菌属,菌株2011072708与食物盐单胞菌Halomonas alimentaria有99％的同源性,而菌株2011072709与胜利盐单胞菌H.shengliensis有100％的同源性.比较研究了两株菌在不同温度、盐度、pH、碳氮比条件下对氨氮的转化率,菌株2011072708在温度37℃、盐度30～40、pH 8、碳氮比28的条件下对氨氮的转化率最高;菌株2011072709在温度27～42℃、盐度40～50、pH 6、碳氮比21的条件下对氨氮的转化率最高.研究结果表明,胜利盐单胞菌（2011072709）对温度、盐度、pH、碳氮比等各方面的适应性优于食物盐单胞菌（2011072708）,更适合在生物絮团技术中得到应用.Two strains of nitrosobacteria with high ammonia transformation rate were isolated by enrichment and selective culture from bio-flocs of shrimp culture ponds.Sequences of 16S rRNA indicated that both strains belonged to the genus Halomonas sp.,while strain 2011072708 was identified as H.alimentaria with 99% similarity,and strain 2011072709 as H.shengliensis with 100% similarity.Ammonia transformation rates of the two strains were compared under different temperatures,salinities,pH,and C/N ratios.The ammonia transformation rate of strain 2011072708 reached the highest level at 37 ℃,30～40,pH 8,and C/N ratio of 28,while that of strain 2011072709 reached the maximum at 27～42 ℃,40～50,pH 6,and C/N ratio of 21.The present study showed high ammonia transformation rates in both nitrosobacteria strains isolated from bio-flocs.Strain H.shenliensis （2011072709） had better adaptability to temperature,salinity,pH,and C/N ratio than H.alimentaria （2011072708）,and may be more suitable for application in bio-flocs technology.

关 键 词：生物絮团 异养亚硝化细菌 氨氮转化率 盐单胞菌 

分 类 号：Q939.9[生物学—微生物学]