MMP-2、MMP-3、MMP-9和TIMP-1评价膝关节骨性关节炎的临床研究  

The value of MMP-2,MMP-3,MMP-9 and TIMP-1 levels in the evaluation of knee joint osteoarthritis

作　　者：贺占坤[1] 沈杰威[2] 

机构地区：[1]河南省新乡市第一人民医院骨科,453000 [2]北京积水潭医院骨科,北京100035

出　　处：《重庆医学》2013年第32期3872-3874,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(81101397)

摘　　要：目的研究膝关节骨性关节炎(OA)患者关节液中基质金属蛋白酶(MMP)-2、MMP-3、MMP-9和基质金属蛋白酶抑制剂-1(TIMP-1)4种蛋白水平,并探讨其与关节损伤程度及患者预后的关系。方法 52例患者均给予关节镜下清理术联合玻璃酸钠、双醋瑞因的治疗方法,于治疗前后采用ELISA检测52例膝关节OA患者及10例症状轻、X线等影像阴性者关节液中MMP-2、MMP-3、MMP-9和TIMP-1的含量并进行相关分析,并在关节镜下对膝关节软骨损伤程度进行评价。结果膝关节OA患者关节液中MMP-2、MMP-3、MMP-9和TIMP-1的含量均明显高于对照组(P<0.01)。关节液中MMP-2、MMP-3、MMP-9和TIMP-1的含量与关节软骨损伤程度呈正相关,随着病情的好转,关节液中MMP-2、MMP-3、MMP-9和TIMP-1的含量也随之降低。结论检测关节液中MMP-2、MMP-3、MMP-9和TIMP-1水平对膝关节OA的早期诊断、病情程度判断、预后评价有一定的意义。Objective To explore the relationship between the level of matrix metallo-proteinase（MMP）-2, MMP-3, MMP-9 and matrix metallo-proteinase inhibitor-l（TIMP-1） in the synovial fluid of the patients with knee joint osteoarthritis（OA） and the de- gree of articular cartilage injury and prognosis. Methods 52 patients（knee OA group） were given arthroscopic debridement com- bined with sodium hyaluronate,diacerein. The levels of MMP-2, MMP-3, MMP-9 and TIMP-1 in synovial fluid were detected in 52 patients with knee OA and 10 normal controls（control group） by enzyme-linked immunosorbent assay. The degree of cartilage inju- ry was assessed with arthroscopy. Results The level of MMP-2,MMP-3,MMP-9 and TIMP-1 in knee OA group were significantly higher than those of the control group（all P^0.01）. The levels of MMP-2, MMP-3, MMP-9 and TIMP-1 in the synovial fluid were positively correlated with the degree of articular cartilage injury,and the levels of MMP-2, MMP-3,MMP-9 and TIMP 1 decreased with the improvement of the disease. Conclusion The measurement of MMP-2, MMP-3, MMP-9 and TIMP-1 have a certain signifi- cance to early diagnosis,degree judgment and prognostic evaluation of knee OA.

关 键 词：骨关节炎 滑液 膝关节 基质金属蛋白酶 预后 

分 类 号：R684[医药卫生—骨科学]