猪圆环病毒2型辽宁株全基因组克隆与序列分析  

Cloning and Sequence Analysis of Complete Genome of Porcine Circovirus Type 2(PCV2)Liaoning Strain

作　　者：隋昀原[1] 荣柳 陈美君[1] 朱耀才[1] 沈国顺[1] 

机构地区：[1]沈阳农业大学畜牧兽医学院,辽宁沈阳110866

出　　处：《中国畜牧兽医》2013年第11期26-29,共4页China Animal Husbandry & Veterinary Medicine

基　　金：辽宁省科技计划项目(2011215014)

摘　　要：本试验从辽宁地区某猪场疑似患有断奶仔猪多系统衰竭综合征（PMWS）的猪群中采集病料，采用PCR方法进行猪圆环病毒2型（porcinecircovirustype2，PCV2）的检测，在此基础上对阳性病料进行PCV2全基因组克隆和序列分析。结果表明，PCV2辽宁分离株基因组全长为1768bp，与国内外PCV2分离株的同源性为95．9％～99．5％，其中与丹麦分离株（EF565365）、澳大利亚分离株（AY424405）和江苏分离株（FJ158606）同源性最高，均为99．5％；与广东分离株（JX912915）同源性最低，为95．9％。In this study, some PMWS-suspected samples from a swine farm in Liaoning province were detected by PCR method, then we amplified the complete genome of PCV2 and made some sequence analysis. The results showed that the com- plete genome of PCV2 Liaoning strain was 1768 bp in length. The nucleotide homology between the PCV2 Liaoning strain and other strains ranged from 95.9% to 99.5%. Among these strains, the PCV2 Liaoning strain showed the highest homology with Denmark （EF565365）, Australia （AY424405） and Jiangsu （FJ158606）, all about 99.5%, the lowest homology was with Guangdong （JX912915） about 95.9%.

关 键 词：猪圆环病毒2型 全基因 克隆 序列分析 

分 类 号：Q781[生物学—分子生物学]