布莱凯特黑牛心脏脂肪酸结合蛋白基因单核苷酸多态性的研究  

Study on Single Nucleotide Polymorphisms of H-FABP Gene in Black Cattle

作　　者：赵仕全[1,2] 董雅娟[1,2,3] 柏学进[1,2,3] 牛召珊[1,2] 杨莉[1,2] 张新浩[1,2] 

机构地区：[1]青岛农业大学动物胚胎工程中心,山东青岛266109 [2]山东省黑牛繁育工程技术研究中心,山东青岛266109 [3]山东布莱凯特黑牛科技股份有限公司,山东淄博256306

出　　处：《中国畜牧兽医》2013年第11期42-45,共4页China Animal Husbandry & Veterinary Medicine

基　　金：山东省良种工程(2010LZ012-03)

摘　　要：利用PCR-SSCP方法测定96头布莱凯特黑牛H-FABP基因外显子2和内含子2的部分序列多态性,并对含有多态序列的片段进行了序列分析。结果表明,引物1、3的扩增序列不存在多态性,引物2、4的扩增序列存在多态性;引物2在内含子第323位点处存在G→A突变,在群体中产生了AA、AB、BB 3种基因型,基因型频率分别为0.448、0.146、0.406,多态信息含量(PIC)为0.375,属于中度多态(0.25<PIC<0.5),经χ2检验该位点基因型频率不处于Hardy-Weinberg平衡状态;引物4在内含子2第872位点处存在C→G的突变,产生了HH、Hh、hh 3种基因型,基因型频率分别为0.104、0.417、0.479,多态信息含量(PIC)为0.337,为中度多态(0.25<PIC<0.5),经χ2检验该位点基因型频率达到Hardy-Weinberg平衡状态。The single nucleotide polymorphisms（SNPs） in exon 2 and part of intron 2 of H-FABP gene in 96 individuals from Black cattle were detected by PCR-SSCP methods and sequencing analysis technique. The results showed that there were two SNPs in intron 2 at primer 2 and primer 4, no variance at primer 1 and primer 3. Three genotypes （AA, AB, BB） deter- mined by SNP at 323 （G→A） were detected using primer 2. Their genotype frequencies were 0. 448, 0. 146, 0. 406, which were no in Hardy-Weinberg equilibrium with chi-aquare analysis. Statistical analysis revealed an intermediate polymorphism in- formation content （PIC） in Black cattle. Three genotypes （HH, Hh, hh） determined by SNP at 872 （C→G） were detected using primer 4. Their genotype frequencies were 0. 104, 0. 417, 0. 479, which were in Hardy-Weinberg equilibrium with chi- aquare analysis. Statistical analysis revealed an intermediate polymorphism information content （PIC） in Black cattle.

关 键 词：布莱凯特黑牛 心脏脂肪酸结合蛋白基因 单核苷酸多态性 

分 类 号：Q349.53[生物学—遗传学]