长白猪雌激素受体和卵泡刺激素β基因多态性对繁殖性能的影响  

Effects of the Polymorphisms of ESRand FSHβGenes on Reproductive Traits in Landrace Pigs

作　　者：王靖[1] 赵乔辉[1] 朱世平[1] 吴正常[1] 程洪青 包文斌[1,2] 吴圣龙[1,2] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]扬州市种猪繁育工程技术研究中心,江苏扬州225009

出　　处：《中国畜牧兽医》2013年第11期129-133,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家星火计划重点项目(2012GA690003);江苏省科技支撑计划(农业)(BE2010371;BE2012330);扬州市农业科技攻关项目(YZ2011048;YZ2011153)

摘　　要：本研究以84头长白猪为试验材料,采用PCR-RFLP方法检测雌激素受体(estrogen receptor,ESR)和卵泡刺激素β(follicle-stimulating hormoneβ,FSHβ)基因的多态性,并进一步分析这2个基因多态位点及其合并基因型对繁殖性能的影响。结果表明,ESR和FSHβ基因均存在AA、AB和BB 3种基因型,2基因座均处于Hardy-Weinberg平衡状态;单基因效应分析结果表明,ESR基因AB基因型个体部分繁殖性能指标显著高于AA基因型,AB基因型为ESR基因的有利基因型。初产母猪FSHβ基因中BB、AA、AB基因型个体的所有繁殖性能指标均呈递增趋势,而经产母猪中AA基因型个体的性能指标较高,但是差异不显著(P>0.05);ESR与FSHβ的合并效应分析结果表明,基因聚合效应并不是各个基因效应的简单相加,在繁殖性能已经稳定的第3胎次,AA-AB型个体的繁殖性能显著低于其他合并基因型个体,表明该基因型为不利的合并基因型,故在长白猪选种选育过程中应优先考虑被淘汰。The polymorphisms of estrogen receptor （ESR） and follicle-stimulating hormone β （FSHβ） genes in 84 Landrace pigs were detected with PCR-RFLP method and the relationship between reproductive performance and different genotypes （in- clude single gene and combined gene） in this study. The results showed that ESR and FSHβ genes could be divided into AA, AB and BB genotypes, and the pattern types of ESR and FSHβ genes were fit with Hardy-Weinberg equilibrium. Single gene analysis showed that reproductive performances with AB genotype were significantly higher than AA genotype in ESR gene, and AB genotype was the beneficial genotype in ESR gene. In first farrowing sow, the reproductive performances with BB, AA and AB genotypes increased gradually, and in multiparous sow, reproductive performances with AA genotype were higher than others, but the difference was not significant. Combined gene analysis showed that the effect of combined genotype was not simple sum of single genotype. In the third parity which reproductive performances had already stable, reproductive perform- ances with AA-AB combined genotype were lower than other combined genotypes. So this combined genotype was detrimental combined genotype and should be culled in marker-assisted selection.

关 键 词：雌激素受体基因 卵泡刺激素β基因 合并基因型 繁殖性能 

分 类 号：S814.8[农业科学—畜牧学]