伪狂犬病病毒Ra株对不同细胞增殖特性及致病性研究  

Study on Proliferation Characteristics and Pathogenicity of Pseudorabies Virus Ra Strain in Different Cells

作　　者：余文兰[1] 张晓战[1] 黄红亮 刘碧涛[1] 任常宝 唐兆新[1,2] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]肇庆大华农生物药品有限公司,广东肇庆526238

出　　处：《中国畜牧兽医》2013年第11期164-168,共5页China Animal Husbandry & Veterinary Medicine

基　　金：广东省肇庆市产学研结合项目(00074971221021021)

摘　　要：本研究以伪狂犬病病毒(pseudorabies virus,PRV)Ra株体外感染不同的细胞系为模型,研究PRV Ra株最适细胞系。试验采用ST细胞、PK-15细胞、Vero细胞、F81细胞、DF1细胞、MDCK细胞和CEF细胞7种细胞作为该毒株的接种对象,观察毒株在这几种细胞上病变特点、细胞病变时间及病毒增殖规律等并进行比较。观察结果发现,受试的各种细胞在PRV感染期间均能表现明显的细胞病变,但不同细胞在病变时间上存在较大差异,其中PK-15和ST细胞在感染后24h内出现明显细胞病变,时间最早。TCID50测定病毒增殖力结果表明,ST细胞在病毒增殖传代中毒力保持最好,与原毒株毒力相当,为106.9 TCID50/0.1mL。本试验结果表明,ST细胞是较适合于PRV Ra株体外研究的细胞系。To select the most suitable cell line for research on the pathogenic mechanism and vaccine of pseudorabies virus （PRV） Ra strain, different kinds of cells （ST, PK-15, Veto, F81, DF1, MDCK and CEF） were chosen to be inoculated with PRV. Cytopathy effect （CPE） charaeteristics, time and titers of the cell cultures were compared in this study. According to the morphology observation of infected cells, we found that the PRV Ra strain could proliferate in these seven kinds of cells and cause significant CPE, and the CPE obviously appeared firstly within 24 h on the PK-15 and ST cells. The TCID50 of PRV in the seven kinds of cell cultures performed differently, and the TCID50 of infected ST cell was the highest. Therefore, it sugges- ted that ST cell could be used in the further study of PRV Ra strain in vitro.

关 键 词：伪狂犬病病毒Ra株 细胞系 细胞病变效应 病毒滴度 

分 类 号：S852.65[农业科学—基础兽医学]