滇重楼内生真菌分离与多样性研究  

Isolation and diversity analyses of endophytic fungi from Paris polyphylla var.yunnanensis

作　　者：王茜[1] 申仕康[1] 张爱丽[1] 吴春妍[1] 吴富勤[1] 张新军[1] 王跃华[1] 

机构地区：[1]云南大学生命科学学院,云南昆明650091

出　　处：《中国中药杂志》2013年第22期3838-3844,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(31070306);云南大学生命科学学院科学研究与人才培养开放基金项目(2012S202)

摘　　要：通过研究滇重楼内生真菌菌群的多样性,为探讨内生真菌作为生物活性物质的价值提供科学依据。该文通过对云南省嵩明县、鹤庆县、保山市野生滇重楼植株的采集,从其根、块茎、叶中分离内生真菌,并基于ITS序列分析和形态学方法进行分类鉴定。试验分离得到内生真菌749株,分别归属于41个属,其中3个属仅在根部出现,12个属仅在块茎出现,8个属仅在叶部出现。各个样地分离出的内生真菌表现出一定的差异,其中鹤庆样地块茎部位分离的内生真菌多样性最高,各样地间,各部位间的内生真菌组成相似性系数低。研究认为,不同样地、不同部位的滇重楼内生真菌的差异性可能与生境以及各部位结构、成分等因素有关。The paper is aimed at studying the diversity of endophytic fungi community from Paris polyphyUa var. yunnanensis, and to provide a scientific basis for the utilization value of the endophytic fungi as bioactive material resources. In the present study, endophytic fungi were isolated from roots, rhizomes and leaves of wild P. polyphylla var. yunnanensis collected from Baoshan, Heqing county and Songming city of Yunnan province, and identified and classified by morphological methods together with its ITS sequence analysis. Seven and forty-nine strains of endophytic fungi were isolated from P. polyphylla var. yunnanensis. They were identified be- longing to 41 genus. In these 41 genus, 3 genus exist in root only, 12 genus only exist in rhizome and 8 genus only exist in leaf. There was difference in endophytic fungi isolated from different sample sites. Endophytic fungi diversity from rhizomes of Heqing site was the highest. Endophytic fungi similarity coefficient was low among different sites and tissues. Based on these results, it is reasonable to propose that endophytic fungi of P. polyphylla var. yunnanensis from different tissue and different sample sites has a certain difference which is possibly relate to their different habitats, different structure and composition of each tissue.

关 键 词：滇重楼 内生真菌 分离 鉴定 多样性 

分 类 号：R284.1[医药卫生—中药学]