炮制前后山茱萸煎液、溶出层面的比较研究  

Comparative study on decoction and dissolution of crude and processed Corni Fructus

作　　者：周函钰 杨培培[1,2] 丛晓东[1,2] 张程荣[1,2] 蔡宝昌[1,3] 

机构地区：[1]浙江中医药大学中药炮制技术中心,浙江杭州310053 [2]浙江省省级药学类工程实践基地,浙江杭州310053 [3]江苏省中药炮制重点实验室,江苏南京210049

出　　处：《中国中药杂志》2013年第22期3888-3892,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81274056);浙江省中医药科技计划项目(2009CB008)

摘　　要：从煎液、溶出层面对炮制前后山茱萸中活性成分进行比较研究。采用HPLC,中药煎出法,溶出度法对炮制前后山茱萸的活性成分马钱苷、莫诺苷的煎出率与溶出度特征进行比较研究。结果表明,炮制前后活性成分马钱苷、莫诺苷含量变化不明显;与生品相比,制品(煎液)中马钱苷的含量增高明显,而莫诺苷变化不明显;与生品相比,溶出度层面,在肠液中,制品(提取物)马钱苷的溶出度差别不明显,而莫诺苷溶出度增大、差别明显;在胃液中,制品马钱苷、莫诺苷的溶出度均减少、差别明显,但综合考虑临床上服用的煎药剂量,添加煎出率指标后,煎出溶出积=煎出率×溶出度,则在肠液与胃液中,制品的煎出溶出积指标均增大,差别非常明显,提示制品在临床应用方面优于生品。该文提出从煎液、溶出层面比较炮制前后活性成分变化和煎出溶出积指标,用于山茱萸炮制后的质量评价。To compare and study the decoction and dissolution of active constituents in crude and processed Comi Fructus. HPLC, the traditional Chinese medicine （TCM） decoction method and the dissolution methods were adopted to compare and study the decoction yield and dissolution rate of loganin and morroniside, active constituents in crude and processed Corui Fructus. The results showed that the content of active constituents ]oganin and morroniside in crude and processed Corni Fructus did not change significant- ly; compared with crude Comi Fructus, processed Comi Fructus （decoction） contained much higher loganin, with no obvious change in morroniside; compared with crude Comi Fructus, processed Comi Fructus （extracts） showed no significant difference in loganin dis- solution, but notable increase in morroniside dissolution in intestinal fluid; in gastric fluid, processed Corui Fructus showed significant increase in loganin and morroniside dissolutions. However, in comprehensive consideration of the decoction dose in clinical administra- tion, and calculated on the basis of the forumla of the decoction yield ~ dissolution rate = decoction-dissolution product, it showed in- crease in the decoction-dissolution products of both of the active constituents loganin and morroniside, with significant difference. This suggested that processed Corui Fructus is superior to crude Comi Fructus in clinical application. In this article, we proposed to compare the changes in decoction and dissolution of active constituents in crude and processed Comi Fructus, study the decoction-dissolution product, and then apply it in the quality evaluation of crude and processed Corni Fructus.

关 键 词：山茱萸 炮制 煎出溶出积 煎出率 溶出度 马钱苷 莫诺苷 

分 类 号：R284[医药卫生—中药学]