掌叶半夏(虎掌南星)矾制前后致炎毒性比较研究  

Comparative study on pro-inflammatory toxicity of Pinellia pedatiecta before and after being processed with alum

作　　者：郁红礼[1,2,3] 张倩[1] 吴皓[1,2,3] 邵丞 赵腾斐[1] 李卓[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210029 [2]江苏省中药炮制重点实验室,江苏南京210029 [3]国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210029

出　　处：《中国中药杂志》2013年第22期3893-3897,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30973939;81173549);教育部博士点教师基金项目(20113237120010);南京中医药大学中药学江苏省优势学科开放课题项目(2011ZYX2-012)

摘　　要：目的:探索掌叶半夏致炎毒性及白矾炮制对其致炎作用的影响。方法:自掌叶半夏鲜品中分离毒针晶及掌叶半夏凝集素蛋白;采用整体动物及细胞水平模型,以炎症介质为指标,考察掌叶半夏毒性成分毒针晶和凝集素蛋白的致炎效应,及矾制对致炎作用的影响。结果:腹腔注射掌叶半夏毒针晶混悬液可使小鼠腹腔渗出液中炎症介质PGE2,NO含量显著增加,矾制后生品及毒针晶诱导的PGE2,NO释放显著下降;掌叶半夏凝集素蛋白刺激小鼠巨噬细胞后,在一定时间内可导致剂量依赖性的炎症因子TNF-α及IL-6含量显著增加。矾制后凝集素蛋白诱导的小鼠腹腔渗出液中PGE2含量显著下降。结论:掌叶半夏的刺激性毒性在机体表现为炎症反应,其所含的毒针晶及凝集素蛋白是其毒性成分,均可导致显著的炎症介质释放。矾制可显著降低掌叶半夏毒性成分的致炎毒性。Objective： To explore the pro-inflammatory toxicity of Pinellia pedatiecta, as well as the alum processing method on its pro-inflammatory effect. Method： Raphide and agglutinin （PPA） proteins were isolated from fresh P. pedatiecta. The overall animal and cellular level models were applied to investigate the pro-inflammatory effect of raphide and PPA in P. pedatiecta, as well as the impact of the alum processing method on the pro-inflammatory effect, with inflammatory mediators as the index. Result： Intraper- itoneal injection with P. pedatiecta raphide suspension could significantly increase the content of inflammatory mediators PGE2 and NO. After the alum processing method was adopted, fresh P. pedatiecta and raphide-induced PGE2 and NO release significantly reduced. The stimulation of mice macrophages with P. pedatiecta agglutinin protein could cause the content of dose-dependent inflammatory me- diators TNF-a and IL-6. After the alum processing method was adopted, PGE2 content in P. pedatiecta agglutinin protein-induced mice peritoneal exudate notably decreased. Conclusion： The irritation and toxicity of P. pedatiecta were inflammatory responses in organ- isms. Its raphide and agglutinin proteins were toxic components, both could cause significant the release of inflammatory medium. The alum processing method could help significantly reduce the pro-inflammatory toxicity of P. pedatiecta.

关 键 词：掌叶半夏 毒针晶 凝集素蛋白 致炎作用 白矾炮制 

分 类 号：R285[医药卫生—中药学]