多指标测定优化聚酰胺树脂分离甘草苷的工艺  

Multi-index determination and optimization of liquirtin separated from polyamide resin

作　　者：郑云枫[1] 杨锦强[1] 魏娟花[1] 黄利[1] 彭国平[1] 

机构地区：[1]南京中医药大学中药复方分离工程重点实验室,江苏南京210029

出　　处：《中国中药杂志》2013年第22期3902-3906,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81202881);江苏省自然科学基金项目(SBK201123752)

摘　　要：通过聚酰胺树脂静态、动态吸附及解吸附试验,以甘草苷、异甘草苷及甘草酸3种成分为考察指标,研究优化了甘草中甘草苷的分离工艺,优化的工艺条件为:药液pH 7.0,药液中甘草苷质量浓度1.296 g·L-1,上样体积3 BV,吸附后的树脂分别以水,10%,20%,30%乙醇(各3 BV)进行洗脱,收集20%乙醇洗脱部分,回收溶剂。结果显示,在此工艺条件下分离获得的提取物中异甘草苷、甘草酸等杂质成分含量较低,甘草苷纯度由4.86%提升到88.5%。该方法简单可行,获得的甘草苷提取物纯度较高,可为甘草苷的工业化分离制备提供依据。To optimize the separation process of liquirtin from glycyrrhiz by static, dynamic adsorption and desorption experi- ments on polyamide resin, with liquirtin, isoliquiritin and glycyrrhizic acid as the study index. The optimum process conditions were that the pH of solution was regulated to be 7.0, the concentration of hquirtin was 1. 296 g ~ L- 1, the volume of loading buffer was 3 BV. After absorption, efforts shall be made to elute resin with water, 10% , 20% , 30% ethanol （3 BV for each） , collect 20% eth- anol eluted fraction, and recover solvents. The results showed lower contents of such impurities as isoliquiritin and isoliquiritin in ex- tracts sepaprated under this process conditions, as well as an increase in purity of liquirtin from 4. 86% to 88.5%. The method was simple and feasible, it could obtain a higher purity in extracts from liquirtin and provide basis for industrialized separation and prepara- tion of liquirtin.

关 键 词：甘草 聚酰胺树脂 甘草苷 异甘草苷 甘草酸 

分 类 号：R284[医药卫生—中药学]