凉血解毒方及拆方对活化T淋巴细胞致角质形成细胞增殖及分泌细胞因子的影响  

Effect of blood cooling and detoxification formula and its dismantled formulae on keratinocyte proliferation induced by activated T lymphocytes

作　　者：解欣然[1] 刘欣[1] 王燕[1] 张璐[1] 李萍[1] 

机构地区：[1]首都医科大学附属北京中医医院北京市中医研究所,北京100010

出　　处：《中国中药杂志》2013年第22期3953-3956,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81072810);北京市中医管理局青年基金项目(QN2012-10)

摘　　要：目的:观察凉血解毒方全方及拆方对活化T淋巴细胞致角质形成细胞增殖和分泌细胞因子的影响。方法:制备大鼠含药血清;将PDB刺激后的T淋巴细胞与Colo-16细胞共同培养,同时加入含药血清作用24 h,采用MTT法检测细胞增殖,采用ELISA法检测细胞因子TNF-α,sICAM-1和IFN-γ。结果:凉血解毒方对活化T淋巴细胞诱导的Colo-16细胞增殖具有明显的抑制作用,其中全方组和凉血组差异显著(P<0.01)。活化T淋巴细胞作用于Colo-16细胞24 h后,sICAM-1,TNF-α,IFN-γ均明显升高,凉血解毒全方和凉血方、解毒方可明显降低sICAM-1,TNF-α,IFN-γ,而全方组药效最为显著。结论:临床治疗银屑病有效中药复方凉血解毒方能够显著抑制活化T淋巴细胞介导的角质形成细胞增殖及细胞因子的分泌。Objective： To observe the effect of the blood cooling and detoxification formula and its dismantled formulae on acti- vated T lymphocytes-induced keratinocyte proliferation and cytokine secretion. Method： Rat drug-containing serum was prepared. PDB-stimulated T lymphocytes and Colo-16 cells were co-cultured, then added with the drug-containing serum, and laid aside for 24 h. The cell proliferation was detected by MTT assay. Cytokines TNF-α, sICAM-1 and IFN-T were detected by ELISA. Result： The blood cooling and detoxification formula has a notable inhibitory effect on activated T lymphocyte-induced Colo-16 keratinocyte proliferation, with a significant difference between the whole formula group and the blood cooling group （ P 〈 0. 01 ）. After activated T lymphocytes had added into Colo-16 cells for 24 hour, sICAM-1, TN-α and IFN-T significantly increased. Though the whole blood cooling and de- toxification formula, the blood cooling formula and the detoxification formula could notably reduce sICAM-1, TNF-α, IFN-T, the whole formula group showed the most significant effect. Conclusion： The blood cooling and detoxification formula, as an effective traditional Chinese medicine compound for clinical treatment of psoriasis, can significantly inhibit activated T lymphocyte-induced keratinocyte proliferation and cytokine secretion.

关 键 词：银屑病 T淋巴细胞 Colo-16细胞 凉血解毒方 

分 类 号：R285.5[医药卫生—中药学]