中药材秦艽主要吸收成分群研究  

Efficient method for analyzing absorbed ingredients of traditional Chinese medicine Genitana Macrophyllae Radix

作　　者：朱金武[1,2] 宋蓓[3] 瞿文生[4] 董延生[4] 俞梦孙[1] 朱晶晶[3] 

机构地区：[1]空军航空医学研究所,北京100142 [2]解放军第四军医大学,陕西西安710032 [3]中国中医科学院中药研究所,北京100700 [4]军事医学科学院毒物药物研究所,北京100850

出　　处：《中国中药杂志》2013年第22期3977-3983,共7页China Journal of Chinese Materia Medica

基　　金：国家“重大新药创制”科技重大专项(2009ZX09308-003,2009ZX09301-005,2009ZX09502-021);中央级公益性科研究院所基本科研业务费专项(22070833)

摘　　要：该研究通过制备大鼠外翻肠囊模型,并对其转运活性进行了确认,以秦艽中的马钱苷酸、当药苦苷、龙胆苦苷、以及当药苷为检测指标,用HPLC对经肠囊主动转运吸收后被吸收进入浆膜层的秦艽提取物中的主要成分群进行了分析,探讨了中药材主要吸收成分群的研究方法。结果表明所采用的大鼠外翻肠囊模型,在实验45 min内,组织结构完整,转运活性良好;HPLC的专属性和线性关系良好,精密度的RSD<5.5%;样品的萃取回收率均在90%以上;在室温条件下,样品的稳定性良好。秦艽提取物经肠囊主动转运后,在浆膜液样品中,检测到了8种主要成分;提取物的浓度对主要成分的吸收具有极其显著影响,而不同肠段对主要成分吸收的影响没有显著差异。由此可见,大鼠外翻肠囊技术可用于某些中药材的吸收成分群的研究,是一种探讨中药材活性成分的有效方法。In present study, a method for analyzing the absorbed ingredients of traditional Chinese medicine QinJiao has been developed. A rat everted gut sac （EGS） model has been established, and the transporting capacity of gut sacs was identified by histo- logical examinations. The ingredients including loganic acid, sweroside, gentiopicroside, and swertiamarian in serosal solution ab- sorbed by active transport of rat everted ileum and jejunum from Qinjiao extraction were determined using an HPLC method. Histologi- cal integrality of the gut sacs remains perfect and the active transport activity of them is normal within 45 min of the experiment. The HPLC method employed in this study presents high specificity and good correlation. The relative standard deviation of precision of this method is less than 5.5%. Extraction recovery of samples is more than 90%. And stability of the samples in room temperature is per- fect. Eight ingredients of Qinjiao absorbed in serosal solution are identified. Furthermore, concentration of Qinjiao extraction signifi- cantly affects accumulated absorption and absorption coefficient of the ingredients. However, there is no significant impact on the accu- mulated absorption and absorption coefficient by diverse of everted gut sections. From above, the EGS techniques might be an efficient method, which can be employed for investigation of absorbed ingredients of Traditional Chinese Medicines.

关 键 词：中药材 秦艽 外翻肠囊 HPLC 吸收成分群 

分 类 号：R285.5[医药卫生—中药学]