藏药镰形棘豆总生物碱提取纯化的研究  

Extraction and purification of total alkaloids formOxytropis falcata

作　　者：詹珺雁[1] 燕珂[2] 杨光明[2] 王栋[2] 蔡宝昌[2] 

机构地区：[1]连云港市第一人民医院,连云港222002 [2]南京中医药大学,江苏省中药炮制重点实验室,国家中医药管理局中药炮制标准重点研究室,国家教育部中药炮制规范化及标准化工程研究中心,南京210023

出　　处：《西北药学杂志》2013年第6期551-555,共5页Northwest Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(编号:30902012)

摘　　要：目的研究藏药镰形棘豆总生物碱的提取纯化的最佳工艺。方法选择物料比、乙醇体积分数、醋酸体积分数和提取温度作为实验的考察因素,以酸性染料比色法测定吸光度为指标,采用正交实验优选镰形棘豆总生物碱的提取纯化最佳工艺。结果最优工艺条件为以5mL·L-1醋酸的体积分数40%乙醇溶液为溶剂,物料比为1∶20,60℃超声提取2次,经732型阳离子交换树脂进行吸附,再以10mL·L-1 NH3·H2O的体积分数60%乙醇溶液超声提取树脂中的生物碱。结论优选的工艺稳定可行,能较好地提取镰形棘豆中的总生物碱,为今后从镰形棘豆中获得总生物碱提供了科学依据。Objective To optimize the conditions for extraction and purification of total alkaloids from Tibetan medicine ,Oxytropis f alcata .Methods On the basis of single factor investigation ,four factors were chosen to optimize the preparing conditions by an orthogonal design ,i .e .the ratio of material to reagent ,the temperature of extraction ,the concentrations of ethanol and HAc . Absorbance determined by acidic dye colorimetry was adopted as index . Results The optimal conditions were as follows ：extracting twice aided by ultrasonic with 40% ethanol solution containing 5 mL · L -1 HAc at 60 ℃ ,the ratio of material to reagent 1∶20 ,purifying by 732 cation exchange resin and then extracting with 60% ethanol solution containing 10 mL · L -1 NH3 · H2 O .Conclusion The optimal method is stable and reproducible and can be used for the extraction and purification of total alkaloids from Tibetan medicine O . f alcata .

关 键 词：镰形棘豆 总生物碱 提取纯化 

分 类 号：R284[医药卫生—中药学]