丹参饮片HPLC指纹图谱研究  

HPLC fingerprint of the decoction pieces of Salvia miltiorrhiza

作　　者：程茜菲[1] 刘银环[1] 许苗苗[1] 常青[1] 崔九成[1] 宋小妹[1] 

机构地区：[1]陕西中医学院,咸阳712046

出　　处：《西北药学杂志》2013年第6期556-558,共3页Northwest Pharmaceutical Journal

基　　金：陕西省中医管理局中医药科研课题(编号:ZY10)

摘　　要：目的建立丹参饮片的高效液相色谱指纹图谱,研究不同产地丹参饮片的质量,建立评价丹参饮片质量优劣的指纹图谱分析方法。方法运用Waters 2695DAD-HPLC高效液相色谱仪,采用Thermo C18(4.6mm×250mm,5μm)色谱柱,以乙腈-4mL·L-1甲酸水进行梯度洗脱,流速为1.0mL·min-1,检测波长为270nm,柱温30℃,通过聚类分析和相似度分析建立10批丹参饮片的指纹图谱。结果 10批丹参饮片中获得19个色谱共有峰,不同来源丹参饮片指纹图谱相似度有一定差别。结论采用HPLC方法建立丹参饮片指纹图谱,方法稳定、可靠、简便,色谱图展现的各峰分离度较好,特征明显,可作为丹参饮片真伪鉴别的标准,为丹参饮片的质量评价提供参考依据。Objective To establish the chromatographic fingerprint of decoction pieces of Salvia miltiorrhiza by HPLC . Methods HPLC was performed ,for 10 batches of decoction pieces of S .miltiorrhiza by using Waters 2695 DAD-HPLC system . In the HPLC ,a Thermo C18 column （4 .6 mm × 250 mm ,5 μm） was used ;A linear elution of acetonitrile-formic acid aqueus solution was adopted at the flow rate of 1 mL · min-1 ;the detection wavelength was 270 nm ;the column temperature was 30 ℃ ;Evaluation was performed by cluster analysis （CA） .Results 19 HPLC common peaks were acquired in the 10 batches of decoction pieces of S .miltiorrhiz a ,and there were some differences among the fingerprints of decoction pieces of S .miltiorrhiz a in different samples .Decoction pieces of S .miltiorrhiza could be divided into five grades through the results of hierarchical cluster analysis . Conclusion The HPLC method for establishing the fingerprint of decoction pieces of S .miltiorrhiza is stable ,reliable ,and simple ,and the obtained chromatogram has good peak separation and obvious peak characteristics ,which could be used as the standard for decoction pieces of S .miltiorrhiz a identification .

关 键 词：丹参饮片 指纹图谱 聚类分析 高效液相色谱法 

分 类 号：R282[医药卫生—中药学]