HPLC法测定复方炔雌醇甲羟孕酮胶囊中3种维生素的含量  

Determination of vitamin A,vitamin D_2 and vitamin E in Compound Ethinyloestradiol and Medroxyprogesterone Capsules by HPLC

作　　者：徐长根 柳小秦 吴昊[2] 傅强[2] 

机构地区：[1]陕西省食品药品检验所,西安710061 [2]西安交通大学药学院,西安710061

出　　处：《西北药学杂志》2013年第6期580-583,共4页Northwest Pharmaceutical Journal

基　　金：国家药典委员会国家药品标准提高研究项目(编号:国药典化发[2010]84)

摘　　要：目的：建立一种同时测定复方炔雌醇甲羟孕酮胶囊中维生素A、维生素D2和维生素E含量的高效液相色谱方法。方法色谱柱为Kromasil C18柱，流动相为甲醇-乙腈（90∶10），检测波长为265 nm。结果维生素A、维生素D2和维生素E检测质量浓度的线性范围分别为0．1～1．0mg·mL -1（r＝0．9997）、0．2～5．0μg·mL -1（r＝0．9997）和0．5～16mg·mL -1（r＝0.9999），平均回收率分别为99．6％，100．0％和100．9％，RSD分别为1．0％，1．1％和0．9％。结论该方法快速、准确、重复性好，可用于复方炔雌醇甲羟孕酮胶囊中3种维生素的含量测定。Objective To develop an HPLC method for the simultaneous determination of vitamin A ,vitamin D2 and vitamin E in Compound Ethinyloestradiol and Medroxyprogesterone Capsules .Methods The determination was performed on a Kromasil C18 column ,with a mobile phase consisted of methanol-acetonitrile （90∶10） ,and the detection wavelength was set at 265 nm . Re-sults The linear range of vitamin A ,vitamin D2 and vitamin E were 0 .1~1 .0 mg · mL -1 （r=0 .999 7） ,0 .2~5 .0μg · mL -1 （r=0 .999 7） and 0 .5~16 mg · mL -1 （r=0 .999 9） ,and the average recovery rate were 99 .6% ,100 .0% and 100 .9% ,with RSD of 1 .0% ,1 .1% and 0 .9% ,respectively .Conclusion The established method is easy to operate and shows good precision and repro-ducibility ,and could be used for the determination of 3 kinds of vitamins in Ethinyloestradiol and Medroxyprogesterone Capsules .

关 键 词：HPLC 复方炔雌醇甲羟孕酮胶囊 维生素A 维生素D2 维生素E 含量 

分 类 号：R927.2[医药卫生—药学]