中药复方胶囊对多发性脑梗死痴呆大鼠的影响  

Influence of Chinese herbal compound caspsules on the model rats of multi-infarct dementia

作　　者：周青罡 李俊[2,3] 舒心莹 朱伟[2] 陈筱[2] 陈兰英[2,3] 

机构地区：[1]江西汇仁药业有限公司,南昌330000 [2]中药固体制剂制造技术国家工程研究中心,南昌330006 [3]江西中医学院,南昌330006

出　　处：《西北药学杂志》2013年第6期596-598,共3页Northwest Pharmaceutical Journal

基　　金：国家科技重大专项:重大新药创制(编号:2011ZX09101-003-02)

摘　　要：目的：观察中药复方胶囊（YSYN）对大鼠多发性脑梗死痴呆（MID）的疗效及作用机制。方法于左侧颈外动脉注入复合血栓诱导剂，复制多发性脑梗死痴呆模型。采用Morris水迷宫法进行学习记忆测试；采用酶联免疫法测定海马组织去甲肾上腺素（NE）水平；采用比色法测定海马组织乙酰胆碱酯酶（AchE）、乙酰胆碱转移酶（CHAT）和谷胱甘肽过氧化物酶（GSH-Px）水平。结果 YSYN能够显著提高多发性脑梗死痴呆大鼠学习记忆能力（P＜0．05）；显著降低多发性脑梗死痴呆大鼠海马组织AchE含量，提高多发性脑梗死痴呆大鼠海马组织CHAT、NE及GSH-Px含量（P＜0．05～0．01）。结论 YSYN能够显著改善多发性脑梗死痴呆大鼠的学习记忆功能，对MID具有较好的保护作用，其作用机制可能是通过调节海马组织内NE、Ach（乙酰胆碱）及GS H （谷胱甘肽）的活性来实现的。Objective To investigate the effect of Chinese herbal compound caspsules （YSYN ） on the model rats of multi-infarct dementia（MID） and its mechanism .Methods The multi-infarct dementia model was induced by injecting compound thrombosis in-ducer into the external carotid artery .Morris water maze test was used to evaluate the abilities of learning-memory ;the level of norepinephrine（NE） in hippocampal tissue were assayed by using enzyme-linked immunsorbent ;the levels of acetylcholinesterase （AchE） ,cholinacetyltrans-ferase（CHAT） and glutathione peroxidase（GSH-Px） in the hippocampal tissue were assayed by using colorimetry .Results The YSYN could significantly improved the abilities of learning-memory in MID rats（P〈0 .05）;descreased the level of AchE in hippocampal tissue ,increased the level of CHAT ,NE and GSH-Px in hippocampal tissue（P〈0 .05-0 .01） . Conclusion The YSYN could significantly improved the abilities of learning-memory in MID rats ,has a good protective effect on MID rats ;its mechanism may be related to adjusting the level of NE ,AchE ,CHAT and GSH-Px in hippocampal tissue .

关 键 词：多发性脑梗死痴呆 YSYN 乙酰胆碱 GSH-PX NE 

分 类 号：R965[医药卫生—药理学]