HCG与LHRHa对雌性蜂巢石斑鱼产卵期间生理变化的影响及比较  

Impacts of artificial maturation inducing hormone HCG and LHRHa on the physiological fluctuation of female honeycomb grouper,Epinephelus merra, during its spawning season and comparison between the two hormones

作　　者：左永松[1,2] 仲地政人 中村将 征矢野清 陈再忠[1] 钟俊生[1] 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]长崎大学环中国东海资源环境研究所 [3]琉球大学热带生物圈研究所

出　　处：《上海海洋大学学报》2013年第6期841-848,共8页Journal of Shanghai Ocean University

基　　金：上海市重点学科水生生物学建设项目(S0701)

摘　　要：选取日本冲绳地区雌性蜂巢石斑鱼为对象,描述与分析了HCG与LHRHa对该鱼催产效果的差异及注射后所引起的性腺发育变化和排卵相关的性类固醇变化,希望能为蜂巢石斑鱼的人工繁殖提供可靠的理论基础,以及在实践生产中的重要参考。结果表明,HCG与LHRHa均显著促进了决定排卵的性成熟诱导物质MIS的释放以及鱼的产卵;产卵后的蜂巢石斑鱼雌二酮E2的水平显著下降,验证了蜂巢石斑鱼卵巢发育及产卵随月亮周期变化的周期性;在渔业生产中注射催产药物前,确定对象鱼卵泡发育阶段对于提高催产成功率十分重要。在LHRHa注射组,完成卵黄化的蜂巢石斑鱼催产效果最佳,卵黄化早期的鱼不产卵;HCG对卵泡发育初期的蜂巢石斑鱼的催产效果优于LHRHa,但催产效果仍不理想,而对于完成了卵黄化鱼的催产效果不如LHRHa。结合LHRHa的其他优势,本研究认为在具备检验鱼体卵巢发育阶段实验技术的条件下,更加推荐LHRHa作为雌性蜂巢石斑鱼的催产药物。Female honeycomb grouper, Epinephelus merra, was selected as research object in this study. Artificial hormones HCG and LHRHa were used for the description and analysis of gonad developmental changes and sexual steroids fluctuation following hormone injection. Purpose of this study is to establish reliable fundamental information for artificial reproduction of honeycomb grouper, and offer significant reference on manipulation practice. According to the results, both the hormones HCG and LHRHa significantly induced spawning and the releasing of MIS, which determines success of ovulation; Estradiol level of honeycomb grouper decreased significantly after spawning identifying the lunar related periodicity of the ovary development in honeycomb grouper species; In fishery productive practice, it is very important to confirm the ovary developmental stage before hormone injection in order to enhance success of inducing maturation, especially for LHRHa injection of which the effectiveness of inducing maturation was the best; As for honeycomb grouper with most oocytes that were in early stage of development, effectiveness of HCG was higher than that of LHRHa, but it was still unsatisfying. Considering the best effectiveness for the ovaries which completed vitellogenesis in LHRHa treatment group and its other advantages over HCG, we highly recommend the application of LHRHa in honeybomb grouper reproductive industry.

关 键 词：蜂巢石斑鱼 绒毛膜促性腺激素 促黄体激素释放激素类似物 雌二醇 性成熟诱导物质 

分 类 号：Q492.5[生物学—生理学] S917[农业科学—水产科学]