2 939例临床患者CD4^+CD8^+T淋巴细胞检测结果的回顾性分析  

Retrospective Analysis on CD4^+CD^8+ T Lymphocytes Percentages in 2 939 Clinical Cases

作　　者：唐甜[1] 张子宁[1] 傅雅静[1] 王亚男[1] 姜拥军[1] 耿文清[1] 郭晓临[1] 尚红[1] 

机构地区：[1]中国医科大学附属第一医院检验科卫生部艾滋病免疫学重点实验室,沈阳110001

出　　处：《中国医科大学学报》2013年第11期978-981,共4页Journal of China Medical University

基　　金：国家科技重大专项课题(2012ZX10001 006);卫生部项目(中国人群重要常规临床检验项目参考区间的建立)

摘　　要：目的回顾分析中国医科大学附属第一医院2 939例临床患者外周血CD4+CD8+双阳性(DP)T细胞在不同疾病和年龄中异常的比例,初步探讨这群细胞对疾病辅助诊断的意义。方法应用流式细胞术检测并比较临床患者CD4+CD8+DP T细胞亚群百分含量的差异,CD4+CD8+DP T细胞百分含量>5%判定为异常。结果患呼吸、风湿免疫、肿瘤、感染、肾脏、皮肤等系统疾病的患者CD4+CD8+DP T细胞异常的比例高于健康对照;这些CD4+CD8+DP T细胞异常的患者中按年龄分组,高年龄组(>65岁)CD4+CD8+DP T细胞异常的比例显著高于低年龄组(≤65岁)(P<0.001),而健康对照CD4+CD8+DP T细胞异常的人群中,不同年龄组无统计学差异(P>0.05)。结论呼吸、风湿免疫、肿瘤、感染、肾脏、皮肤等系统疾病中CD4+CD8+DP T细胞异常的比例增加,且这些疾病的患者随着年龄的升高CD4+CD8+DP T细胞异常的比例亦增加(尤其患呼吸系统疾病和肿瘤的患者)。Objective To retrospectively analyze 2 939 patients admitted in the First Hospital of China Medical University about the increased pro- porlion of CD4＊CD8＋ （ double positive, DP ）T cell among different diseases and ages, so as preliminarily explore the significance of/his sub-populalion on assisting disease diagnosis. Methods The percentage of CD4＋ CD8＋ T cell was detemined by flow eytometer. The proportion of CD4＋CD8＋ T cell rnore than 5% was considered to be abnomlal. Results The abnormal proportion of CD4＋CD8＋ T cell was significantly higher in patients with respiratoty diseases, rheumatic diseases, tumor, infer tious diseases, kidney diseases arid demmtosis than healthy controls. In clinical patients, the ab- normal proportion of CD4＋CD8＋ T cell was observed in older patients （ 〉65 years ） compared with younger patients （ ≤ 65 years ） （ P 〈 0.001 ）. There was no significant difterenee in the abnormal proportion of CD4＋CD8＋ T cell in healthy controls with different ages （ P 〉 0.05 ）. Conclusion The ab- normal proportion of CD4＋CD86 T eell was significantly higher in mspiratory diseases, rheumatic diseases, tumor, inteetious diseases, kidney diseas- es mid dennatosis. 331e proportion of CD4＋CD8＋ T cell was increased with the growth of age in chnical patients, in particular, who were suffering from respiratory diseases and cancer.

关 键 词：T淋巴细胞亚群 CD4+CD8+T细胞 疾病 年龄 

分 类 号：R392[医药卫生—免疫学]