维康醇对肝癌细胞增殖抑制和诱导凋亡的作用  

Effects of Alternol on the Cell Proliferation and Apoptosis in Human Hepatocellular Carcinoma Cells

作　　者：王艳丽[1] 朱晓琳[1] 段丽丽 陈杰鹏 牛哲峰[1] 曲迎春[1] 丛培芳[1] 李岭[3] 张美侠[1] 

机构地区：[1]中国医科大学药学院临床药理教研室,沈阳110001 [2]广东汕头保税区双骏生物科技有限公司应用生物技术研究所,广东汕头515071 [3]四川大学生命科学学院遗传医学研究所,成都610041

出　　处：《中国医科大学学报》2013年第11期999-1002,共4页Journal of China Medical University

基　　金：辽宁省自然科学基金(2013021081);重庆市自然科学基金(cstc2013jcyjA1587)

摘　　要：目的研究维康醇对肝癌细胞增殖、细胞周期和细胞凋亡作用的影响。方法以不同浓度维康醇处理对数生长期人肝癌细胞HepG2,利用MTT法测定维康醇对细胞的增殖抑制作用,流式细胞仪PI单染检测细胞周期分布情况的改变,流式细胞仪Annexin V FITC/PI双标记法检测细胞凋亡率。结果 MTT结果显示:维康醇对HepG2细胞具有明显的生长抑制作用并呈浓度和时间依赖性。流式细胞术结果显示:30μmol/L维康醇作用于HepG2细胞48 h后,处于G2/M期的细胞比例显著升高,同时伴随G1/G0期细胞比例下降,与对照组相比差异有统计学意义(P<0.05)。Annexin V FITC/PI染色检测结果显示:30μmol/L维康醇作用于HepG2细胞48 h后,细胞凋亡率显著升高,与对照组相比差异有统计学意义(P<0.05)。结论维康醇对肝癌细胞有明显的增殖抑制作用,并具有浓度和时间依赖性;维康醇通过引起细胞周期阻滞、诱导细胞凋亡发挥其抗肝癌作用。Objective To investigate the in vitro effects of ahemol on cell proliferation and apoptosis of human hepatocellular carcinoma cells. Methods HepG2 cells were treated with different concentrations of altemol. The inhibitory rate of cell proliferation was measured by MTF assay. Cell cycles and apoptosis were evaluated using flow cytometty. Results Compared to solvent control, the proliferation of HepG2 cells was inhibited by altemol in a close and time dependent manner （ P 〈 0.05 ）. After 48 h treatmenl, flow cytometry analysis showed that altemol treatment had result- ed in accumulation of cells at C, dM phases and induced apoptosis of HepG2 cells. Conolusion Altemol may be effective for the treatment ofbepato- cellular carcinoma through its cell cycle arrest and apoptosis induction effects.

关 键 词：维康醇 肝癌 凋亡 细胞周期 

分 类 号：R966[医药卫生—药理学]