半夏泻心汤对糖尿病大鼠小肠运动的影响及其作用机制研究  

Effects of Banxia Xiexin decoction on the small intestinal motility and its mechanisms in diabetic rats

作　　者：蒋楠[1] 余跃[1] 陈凤琴[1] 袁莉莉[1] 王巧民[1] 

机构地区：[1]安徽医科大学附属省立医院,安徽合肥230001

出　　处：《时珍国医国药》2013年第11期2631-2634,共4页Lishizhen Medicine and Materia Medica Research

基　　金：安徽省自然科学基金(No.070413142);安徽省教育厅自然科学研究重点项目(No.KJ2010A192)

摘　　要：目的初步研究半夏泻心汤对糖尿病(diabetes mellitus,DM)大鼠小肠运动的影响及其作用机制。方法将SD大鼠分为对照组、DM组、DM+半夏泻心汤组(DM+BX组)、DM+多潘立酮组(DM+Dom组)。半固体营养糊灌胃测定小肠推进率,免疫组化和图像分析对突触素(Syn)、蛋白基因产物9.5(PGP9.5)和c-kit的表达进行比较。结果 DM+BX组、DM+Dom组的小肠推进率较DM组明显提高(P<0.05),但仍低于对照组(P<0.05)。DM+BX组、DM+Dom组的Syn、PGP9.5、c-Kit阳性产物面积和平均光密度值与DM模型组相比均有提升。结论半夏泻心汤可能对受损的DM大鼠小肠肌间神经丛及ICCs有部分恢复作用从而改善糖尿病大鼠的小肠动力障碍。Objective To investigate the effects of Banxia Xiexin decoction on the small intestinal motility disorder in diabetic rats and its mechanisms. Methods 60 healthy male SD rats were divided randomly into Control group （ n = 10） and experimental group （n = 50）. We inected streptozotocin （STZ,55mg/kg） one time in intraperitoneally to establish Diabetic rat models . 45 out of 50 rats were successfully induced. Then the diabetic ratswere devided into diabetes mellitus（DM） group, DM ＋ Banxia Xiexin decoction group （DM ＋ BX group） and DM ＋ domperidone group （DM ＋ Dom group）. DM ＋ BX group were given the Banxia Xiexin decoction 5.4 g · kg^-1·d^-1, DM ＋ Dom group were given the domperidone 5 mg· kg^-1·d^-1, DM and Control group were given equal volume of distilled water at the same time every day. After 8 weeks intervention, all rats were given the semisolid nutrient paste by gavage for intestinal propulsion rate. The expression of synaptophysin, PGP9.5 and c - Kit was analyzed by im- munehistochemistry and quantified by image analyzer. Results Eight weeks after garage, the intestinal propulsion rate was significantly decreased in the DM group compared with DM ＋ BX group and the DM ＋ Dom group （ P 〈 0.05 ）, but were still lower than control group （ P 〈 0.05 ） compared with the DM group , The total area and mean optical density value of synaptophysin positive products in the myenteric plexus were significantly increased in the DM ＋ BX group and the DM ＋ Dom group （P 〈 0.05 ）, but were still lower than those in the control group （ P 〈 0.05） and so did the c - kit and PGP9.5 positive products in the ICCs and myenteric plexus. Conclusion Banxia Xiexin decoction can improve the small intestinal motility disorder by partially reversing ab- normal changes in the intestinal myenteric plexus and ICCs in diabetic rats.

关 键 词：-糖尿病 半夏泻心汤 突触素 蛋白基因产物9 5 CAJAL间质细胞 

分 类 号：R285.5[医药卫生—中药学]