MMP-8-799C/T多态性与心肌梗死发病风险的关联研究  

Association analysis between MMP-8-799C/T polymorphism and the risk of myocardial infarction

作　　者：何叶[1,2] 岑锦明[3] 杨希立[3] 荆霞[1,4] 刘新光[1,4,5] 熊兴东[1,4,5] 

机构地区：[1]广东省医学分子诊断重点实验室,广东东莞523808 [2]广东医学院公共卫生学院 [3]广东省佛山市第一人民医院 [4]广东医学院衰老研究所 [5]广东医学院生物化学与分子生物学教研室

出　　处：《现代预防医学》2013年第23期4385-4388,4392,共5页Modern Preventive Medicine

基　　金：国家自然科学基金(81000143);广东省自然科学基金(S2011010002922;S2012010008219);东莞市科技计划医疗卫生类重点项目(2011105102007);广东省卫生厅科研基金(A2011431;B2009191);广东医学院科技创新基金(STIF201102)

摘　　要：目的探讨MMP-8—799C／T（rsll225395）基因多态性与心肌梗死发病风险的关系，为心肌梗死的预防和治疗提供新思路。方法利用聚合酶链式反应一连接酶检测反应（PCR—LDR）分析技术，对153例心肌梗死的患者和275名对照个体的MMP-8—799C／T（rsll225395）多态位点进行分型，采用非条件逻辑回归分析统计该多态位点与心肌梗死易感的相关性。结果在总研究人群中，MMP-8—799C／T（rsll225395）与心肌梗死无易感相关性（x2=2．195，P=0．334）。而在不吸烟人群中，M舰D-8—799C／T（rsll225395）与心肌梗死易感显著相关（x2=6．757，P=0．034），T等位基因显著增加个体患心肌梗死的发病风险（OR=1．88，95％CI=1．14～3．10，P=0．014）。结论在不吸烟人群中MMP-8—799C／T多态位点与中国汉族心肌梗死遗传易感有关，并且T等位基因可能是心肌梗死的危险因素。OBJECTIVE To investigate the relationship between the MMP-8 -799C/T （rs11225395） polymorphism and the risk for myocardial infarction （MI） in Chinese Han population. METI-IODS The genotypes of the MMP-8-799C/T （rs11225395） polymorphism were determined in 153 myocardial infarction patients and 275 control blood donors by poly- merase chain reaction-ligase detection reaction （PCR-LDR） . Association between the polymorphism and the risk for myocar- dial infarction was evaluated using unconditional Logistic regression analysis. RESULTS In the general population, no signif- icant difference was observed in the frequency of MMP-8 -799C/T genotypes between cases and controls （x2 = 2.195, P = 0.334）. Interestingly, MMP-8 -799C/T polymorphism was associated with the risk of myocardial infarction among non- smokers （x2 = 6.757, P = 0.034）. Significantly increased risk for myoc~,rdial infarction was observed for those carrying T variant allele （OR = 1.88, 95%CI =1.14-3.10, P = 0.014）. CONCLUSION The present study suggests that -799CfF （rs11225395） polymorphism of MMP-8 gene is associated with myocardiai~ infarction in Chinese Han population among non- smokers, and the T allele may be a predictor for the susceptibility of myoc~,rdial infarction.

关 键 词：MMP-8 单核苷酸多态性 心肌梗死 遗传易感 

分 类 号：R349.3[医药卫生—基础医学]