二硫化碳对大鼠睾丸支持-生精共培养细胞凋亡线粒体途径相关基因表达的影响  

Effect of Carbon Disulfide on Expression of Genes Related with Mitochondrion Mediated Sertoli-Germ Cells Apoptosis in Male Rats

作　　者：张振[1] 王为[1] 董煜[1] 黄晓彧[1] 郭寅生[1] 周义军[1] 陈国元[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系,湖北430030

出　　处：《环境与职业医学》2013年第11期870-874,共5页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金资助项目(编号:81172637)

摘　　要：[目的]研究二硫化碳（carbondisulfide，CS：）染毒对大鼠睾丸支持-生精共培养细胞凋亡线粒体途径相关基因表达的影响。[方法]选用22～24d龄清洁级雄性SD大鼠，建立睾丸生精-支持细胞体外共培养体系，将细胞分为4组：1个对照组和3个不同浓度csz染毒组（1、2、4μmol／mL），提取RNA，应用荧光实时定量聚合酶链反应（RT-PCR）检测Cyto C、 Apaf-1、Caspase-3、Caspase-9、Aif、EndoG、Smac、IAPS、 Bax、 Bcl-2 mRNA表达水平。[结果]大鼠睾丸支持一生精共培养细胞中，CS2染毒可导致CytoC、Smac、IAPS、Caspase-3、BaxmRNA的表达水平和Bax／Bcl-2mRNA表达水平值均明显升高（P〈0．05），同时使Apaf-1、Aif、Bcl-2和EndoGmRNA的表达水平明显下降（P〈0．05）。[结论]CS2染毒可影响大鼠睾丸支持-生精共培养细胞凋亡线粒体途径相关基因的表达，并且这一机制与CS2导致生殖损伤相关。[ Objective ] To study the effects of carbon disulfide （CS2） on the expression of genes which are related with mitochondrion mediated sertoli-germ cells apoptosis in male rats. [ Methods ] A 22-24 day-old SD male rat of clean grade was selected to establish the rat sertoli-germ cells co-culture system in vitro. According to the designed concentrations of CS2 （0, 1, 2, 4 μmol/mL）, the cells were divided into four groups accordingly and RNA were extracted. The mRNA expression levels of Cyto C, Apaf-1, Caspase-3, Caspase-9, Aif, EndoG, Smac, lAPS, Bax, Bcl-2 were detected by real-time fluorescent quantitative polymerase chain reaction （RT-PCR）. [ Results ] Comparing with the control group, the levels of Cyto C, Smac, lAPS, Caspase-3, Bax, and Bax/Bcl-2 mRNA in the sertoli-germ cells were increased, while the levels ofApaf-1, Aif, Bcl-2, and EndoG mRNA were decreased in CS2 exposure groups （P〈 0.05）. [ Conclusion ] CS2 exposure affects the expression of genes related with mitochondrial mediated apoptosis, and it probably relates to reproductive toxicity induced by CS2.

关 键 词：二硫化碳 支持-生精细胞共培养 线粒体 细胞凋亡 基因 大鼠 

分 类 号：R114[医药卫生—卫生毒理学]