天麻素的抗糖尿病作用实验  

Research on Anti-Diabetic Efficacy of Gastrodin

作　　者：韩磊[1] 乔爱敏[1] 刘青[1] 

机构地区：[1]华侨大学化工学院,福建厦门361021

出　　处：《华侨大学学报（自然科学版）》2013年第6期682-686,共5页Journal of Huaqiao University(Natural Science)

基　　金：福建省自然科学基金资助项目(2013J01337);华侨大学侨办科研基金资助项目(10QZR15);华侨大学教学改革科研基金资助项目(66661222Y)

摘　　要：分离、培养人脐静脉内皮细胞(HUVECs),测定在高糖刺激下胞外乳酸脱氢酶(LDH)的活性,并用链脲佐菌素建立糖尿病小鼠模型,分别以不同天麻素质量浓度干预14d进行试验.研究结果表明:天麻素能降低高糖刺激下HUVECs胞外LDH的活性,减轻高糖对血管内皮细胞造成的损伤;天麻素能显著降低糖尿病小鼠的血糖,降低小鼠血清中甘油三酯、总胆固醇及丙二醛浓度,提高超氧化物歧化酶(SOD)的活性,显示出明显的抗糖尿病作用.The human umbilical vein endothelial cells （HUVECs） were separated and cultivated. The lactate dehydro- genase （LDH） activity in damaged HUVECs stimulated by high glucose was then determined. The anti-diabetic experi- ment in vivo was performed on streptozotocin （STZ）-induced diabetic mice, and the diabetic mice were treated with gast- rodin （12. 5, 25 and 50 mg · kg^- 1） for 14d. The results showed that the gastrodin could decrease the extracellular LDH activity of HUVECs and reduce the damage of endothelial cells under high glucose. Moreover, gastrodine could decrease the blood glucose of diabetes mice significantly, and the serum triglyceride （TG）, the content of total cholesterol （TC）, and the malondialdehyde （MDA） content were all decreased. It also improved the super oxide dismutase （SOD） activity in diabetic mice serum. Therefore, it can be concluded that the gastrodin has an obvious anti-diabetlc effect in the STZ-in- duced diabetic mouse model.

关 键 词：天麻素 糖尿病 血糖 血脂 抗氧化 

分 类 号：R285.5[医药卫生—中药学] R587.1[医药卫生—中医学]