红花提取物对激素性股骨头缺血坏死兔股骨头组织ERK、JNK和P38蛋白表达的影响  

Effects of Safflower Extract on the Expressions of ERK,JNK and P38 in Femoral Head Tissue of Rabbits With Steroid-Induced Avascular Necrosis

作　　者：万甜[1] 吴敏瑞 齐振熙[2] 

机构地区：[1]福建中医药大学骨伤学院 [2]福建中医药大学中西医结合学院

出　　处：《中医杂志》2013年第23期2039-2042,共4页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(465744)

摘　　要：目的探讨红花提取物对激素性股骨头缺血坏死的可能作用机制。方法将40只成年新西兰大白兔随机分为正常对照组、模型对照组、髓芯减压组、生理盐水+髓芯减压组、红花提取物+髓芯减压组,每组8只。除正常对照组外,其余各组建立激素性股骨头坏死模型。造模成功后,正常对照组和模型对照组不予处理,髓芯减压组仅行髓芯减压术,生理盐水+髓芯减压组采用髓芯减压术配合髓腔内注射生理盐水,红花提取物+髓芯减压组采用髓芯减压术配合髓腔内注射羟基红花黄色素A(7.2mg/kg)。1周后处死兔,取出患侧股骨头,采用免疫印迹法检测组织中ERK、JNK和P38蛋白表达。结果与正常对照组比较,模型对照组ERK1和ERK2表达明显降低,JNK和P38表达明显升高(P<0.05);与模型对照组比较,髓芯减压组、生理盐水+髓芯减压组和红花提取物+髓芯减压组ERK1和ERK2表达明显升高,JNK和P38表达明显降低(P<0.05);与髓芯减压组比较,红花提取物+髓芯减压组ERK1和ERK2表达升高明显,JNK和P38表达降低明显(P<0.05)。结论红花提取物可通过激活丝裂原活化蛋白激酶(MAPK)相关通路,增高ERK的磷酸化水平,降低JNK和P38的磷酸化水平,促进股骨头髓腔内细胞增殖、分化,抑制其凋亡,从而减轻激素导致股骨头缺血坏死的损伤,促进坏死股骨头的修复。Objective To research the possible mechanism of safflower extract for steroid-induced avascular necrosis. Methods Forty adult New Zealand white rabbits were randomized into the normal control group, model control group, core decompression group, normal saline ＋ core decompression group and safflower extract ＋ core decompression group, with 8 in each. The models of steroid-induced avascutar necrosis were established in all groups except the normal control group. There was no treatment in the normal control group and model control group after modeling. The core decompression group was given the core decompression. The normal saline q- core decompression group was given normal saline and core decompression. The safflower extract -/- core decompression group was given 7.2mg/kg safflower yellow A and core decompression. The rabbits were sacrificed one week later. The femoral head in the affected side was removed and the expressions of ERK, JNK and P38 were detected with Western Blotting. Results Comparing with the normal control group, the expressions of ERK1 and ERK2 were significantly decreased but the expressions of JNK and P38 were significantly increased in the model control group （P〈 0.05）. Comparing with the model control group, the expressions of ERK1 and ERK2 were significantly increased but the expressions of JNK and P38 were significantly decreased in the core decompression group, normal saline -t- core decompression group and safflower extract ＋ core decompression group （P〈0.05）. Comparing with the core decompression group, the expressions of ERK1 and ERK2 were significantly increased but the expressions of JNK and P38 were significantly decreased in the safflower extract ＋ core decompression group （P〈 0.05）. Conclusion Safflower extract can reduce the damage and promote the repair of steroid-induced avascular necrosis by activating the mitogen-aetivated protein kinase pathway, increasing the phosphorylation level of ERK, reducing the phosphorylation levels of JNK and P38 and prom

关 键 词：股骨头缺血坏死 红花提取物 羟基红花黄色素A 髓芯减压术 丝裂原活化蛋白激酶 

分 类 号：R285.5[医药卫生—中药学] R274[医药卫生—中医学]