携带bla_(ADC)基因鲍曼不动杆菌可移动遗传元件研究  

Research on the mobile genetic elements in Acinetobacter baumannii carried with bla_(ADC) gene

作　　者：廖梦婷[1] 谷秀梅[1] 陈瑶[1] 李艳华[1] 刘文恩[1] 

机构地区：[1]中南大学湘雅医院检验科,长沙410008

出　　处：《临床检验杂志》2013年第10期740-743,共4页Chinese Journal of Clinical Laboratory Science

基　　金：中南大学大学生创新训练项目(CY12401)

摘　　要：目的了解携带bla ADC基因的鲍曼不动杆菌(Acinetobacter baumannii,Ab)中整合子、转座子、插入序列及接合性质粒等可移动遗传元件的携带情况。方法收集临床分离的179株Ab,用PCR扩增检测bla ADC基因,对bla ADC扩增阳性的菌株用PCR检测4类可移动遗传元件整合子遗传标记(intI 1、intI 2、intI 3、qacE△1-sul1)、转座子遗传标记(merA、tnpU、tnp513、tnpA)、插入序列遗传标记(ISAba1、IS26)及接合性质粒遗传标记(traA、trbC)等基因,并用K-B纸片扩散法对bla ADC扩增阳性菌株分别进行药物敏感性试验。结果 179株Ab中共检出bla ADC基因阳性117株。其中,intI 1、qacE△1-sul1、merA、tnpU、tnp513、ISAba1、IS26检出阳性率分别为71.8%、98.3%、94.9%、93.2%、76.9%、98.3%、100.0%,其余5种可移动遗传元件未检出。每株最少检出可移动遗传元件3种,占总株数的0.9%;最多检出7种,占总株数的50.4%。结论携带bla ADC基因的Ab易检出各类可移动遗传元件,其耐药性可能与可移动元件的存在有关。Objective To investigate the distribution of the mobile genetic elements, including integrons, transposons, insertion sequences and conjugative plasmids, in Acinetobacter baumannii carried with blaADc gene. Methods One hundred and seventy-nine isolates of Acinetobacter baumannii were collected, and their blaADC gene was determined by PCR. Then, four kinds of mobile genetic elements, including integron genetic marks such as intl 1, intl 2, intl 3 and qacE△l-sull, transposon genetic marks such as merA, tnpU, tnpS13 and tnpA, insertion sequence genetic marks such as ISAbal and IS26, and conjunction plasmid genetic marks such as traA and trbC, and the antimicrobial susceptibility of the isolates with blaADc gene were detected by PCR and the disk diffusion method, respectively. Results There was 117 isolates of Acinetobacter baumannii carried with blaADc gene in all 179 isolates. Among them, the positive rates of intll, qacE△1-sull, merA, tnpU, tnpS13, ISAbal and IS26 were 71.8%, 98.3%, 94.9%, 93.2%, 76.9%, 98.3% and 100.0%, respectively, and the other 5 genes were negative. There were at least 3 and at most 7 mobile genetic marks to be detected for each of 117 isolates, and the isolates with 3 and 7 mobile genetic marks accounted for 0.9% and 50.4%, respectively. Conclusion Mobile genetic elements can be easily detected from the Acinetobacter baumannii carried with blaADC, and its drug resistance may be related to the existence of mobile genetic elements.

关 键 词：鲍曼不动杆菌 耐药 blaADC 可移动遗传元件 

分 类 号：R446.5[医药卫生—诊断学]