驱动蛋白分子6基因rs20455位点基因多态性与江苏地区汉族人冠心病的相关性研究  

Association of KIF6 gene rs20455 polymorphisms with coronary heart disease in Han population of Jiangsu area

作　　者：张正芳[1,2] 张葵[2] 魏红霞[2] 李雷[2] 顾光煜[2] 王丽[2] 夏永泉[2] 宋宏岩[2] 

机构地区：[1]江苏大学基础医学与医学技术学院,江苏镇江212003 [2]南京大学医学院附属鼓楼医院检验科,南京210008

出　　处：《临床检验杂志》2013年第10期774-777,共4页Chinese Journal of Clinical Laboratory Science

基　　金：南京市科技发展一般项目(YKK12067)

摘　　要：目的观察江苏地区汉族人群驱动蛋白分子6（KIF6）rs20455位点基因多态性与冠心病（CHD）的关系。方法用聚合酶链反应．限制性片段长度多态性技术（PER—RFLP）分别检测556例冠心痛患者和322例体检健康者K1F6rs20455基因型，计算等位基因分布频率；用全自动生化分析仪检测研究对象血脂、血糖水平。结果冠心病组和健康人对照组KIF6基因rs20455位点TF、TC、cc基因型频率分别为0．291、0．507、0．201和0．323、0．450、0．227（Х^2=2．647，P=0．266）；T、C等位基因频率分剐为0．545、0．455和0．548、0．452（Х^2=0．017，P=0．898）。Logistic回归分析显示，C等位基因不是发生CHD的危险因素（OR=0．862，P=0．326，95％CI为0．641～1．160），年龄、高血压、LDL．C和HbA1c是冠心病发生的主要危险因素（OR分别为1．079、6．239、4．734、11．128，P均〈0．05）。结论KIF6rs20455位点基因多态性与江苏地区汉族人群CHD的发生无关。Objective To investigate the association of kinesin family member 6 （KIF6） gene rs20455 polymorphisms with coronary heart disease （CHD） in Hart population of Jiangsu area. Methods The KIF6 rs20455 genotypes from 556 CHD patients and 322 healthy controls were analyzed by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） techology, and the allele frequencies were calculated. Meanwhile, serum lipids and glucose levels in all participants were measured with an automatic bio- chemistry analyzer. Results The frequencies of TY, TC and CC genotypes were 0. 291, 0. 507 , 0.201 in CHD patients and 0. 323, 0.450, 0. 227 in healthy controls, respectively （Х^2 = 2. 647, P = 0.266）. The allele frequencies of T and C were 0. 545 and 0.455 in CHD patients and 0. 548 and 0. 452 in healthy controls, respectively （Х^2 = 0. 017, P = 0. 898）. The Logistic regression analysis showed that the allele C was not a risk factor for CHD （ OR =0. 862, P =0. 326, 95% CI =0. 641 - 1. 160）, and that age, hypertension, LDL-C and HbAlc were the main risk factors for CHD （ OR = 1. 071, 6. 402, 3. 614 and 10. 175, respectively, P 〈 0.05）. Conclusion KIF6 rs20455 gene polymorphism was not significantly correlated with CHD.

关 键 词：冠心病 驱动蛋白分子6 血脂 基因多态性 

分 类 号：R446.1[医药卫生—诊断学]