翘嘴鳜连续4代选育群体遗传多样性及遗传结构分析  

Genetic structure and genetic diversity analysis of four consecutive breeding generations of Siniperca chuatsi

作　　者：郑荷子 易提林[1] 梁旭方[1] 杨敏[1] 田昌绪 张进[1] 赵程[1] 

机构地区：[1]华中农业大学水产学院农业部淡水生物繁育重点实验室,淡水水产健康养殖湖北省协同创新中心,武汉430070

出　　处：《淡水渔业》2013年第6期8-12,共5页Freshwater Fisheries

基　　金：珠江流域高产精养池塘健康养殖技术集成与示范(2012BAD25B04);国家自然科学基金;鳜驯食性状相关基因功能分析与机理研究(31272641);中央高校基本科研业务费专项(2011PY030)

摘　　要：利用微卫鳜星分子标记技术对翘嘴鳜(Siniperca chuatsi)选育群体世代F1至F4的遗传结构进行分析,并以长江中游野生翘嘴作为对照群体。结果显示:筛选出的7个微卫星位点在5个实验群体中共检测到了149个等位基因;随着选育的进行,4个世代群体遗传多样性参数逐代下降,平均等位基因数从5.14下降到2.57,平均观测杂合度从0.405下降到0.229,平均多态信息含量从0.702下降到0.424。野生群体与选育群体间的遗传距离逐代增加(从0.345 4到0.751 7),遗传相似度逐代减小(从0.707 9到0.471 6),遗传分化指数逐代增大(从0.093 8到0.239 7)。结果表明,经过连续4代选育,部分位点的基因型逐渐趋向纯合,在多数位点上4代群体仍表现出较高遗传多样性。In order to investigate the change of genetic variation among different generations of cultural Siniperca chuatsi populations, we collected four selected generations （ F1, F2, F3 and F4 from fish farms ）, and one wild population in Lushui Reservoir （LS） as control group, and analyzed their genetic variation using seven pairs of microsatellite primers. The result showed that ： a total of 149 alleles were detected in all examined loci. The genetic diversity of the breeding population has been decreasing specifically, the average allele number decreased from 5.14 to 2. 57, the average heterozygosity from 0. 405 to 0. 229, the average polymorphism information content from 0. 702 to 0. 424. The genetic distance between LS and selected generations （F1 to F4 ） increased （from 0. 345 4 to 0. 751 7 ） while the genetic identity decreased （from 0. 707 9 to 0. 471 6） , and the Fst values decreased （from 0. 093 8 to 0. 239 7）. The study suggested that the selective breeding work was effective, tending to result in homozygous and stable population. At the same time, the four selected generations showed high polymorphism at most loci.

关 键 词：翘嘴鳜(Siniperca chuatsi) 选育群体 微卫星 遗传结构 

分 类 号：S917.4[农业科学—水产科学]