植物总DNA提取方法的改进  

Improvement of Extraction Method of Total DNA from Plant

作　　者：王文斗[1] 段英俊[1] 那冬晨[1] 

机构地区：[1]山西师范大学生命科学学院,山西临汾041004

出　　处：《安徽农业科学》2013年第24期9913-9913,9923,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]改进植物总DNA的提取方法。[方法]采用2种研磨方式进行植物DNA提取效果的比较。[结果]2种研磨方式所得DNA质量都较高,可用于后续分子生物学研究;采用研磨缓冲液提取的DNA得率高于液氮研磨。[结论]该方法降低了DNA的提取成本,是一种经济实用的DNA提取方法。[ Objective ] The aim was to improve the extraction method of total DNA from plant. [ Method ] Two kinds of grinding method of DNA extraction were compared in extraction effects. [ Result ] The quality of DNA extracted by the two kinds of grinding method was good, and the DNA could be used in the follow - up molecular biology study ; the DNA extraction yield of grinding buffer grinding was higher than that of liquid nitrogen grinding. [ Conclusion ] The method could reduce the cost of DNA extraction, and was an economical and available method for DNA extraction.

关 键 词：DNA提取 研磨缓冲液 经济实用 

分 类 号：S188[农业科学—农业基础科学]