利用花粉管结合线性DNA的方法将YB2基因导入玉米自交系的研究  

Study on the Transferring Linear of YB2 Gene into Maize Inbred Lines via Pollen Tube Pathway

作　　者：孙会娟[1,2] 李杰[1,2] 邹华文[3] 罗昌[2] 黄丛林[2] 于荣[1] 吴忠义[2] 

机构地区：[1]首都师范大学生命科学学院,北京100048 [2]北京市农林科学院北京农业生物技术研究中心,北京100097 [3]长江大学农学院,湖北荆州434023

出　　处：《安徽农业科学》2013年第24期9918-9920,10016,共4页Journal of Anhui Agricultural Sciences

基　　金：国家转基因重大专项重点课题(2009ZX08003-009B);北京市科委项目(Z121100001512012和Z111100066111005);北京市农林科学院科研能力创新项目(KJCX201102003)

摘　　要：[目的]将YB2基因导入玉米自交系。[方法]利用花粉管通道法将含有YB2基因的线性表达盒转入优良玉米自交系京24,对T0代植物喷洒浓度为200 mg/L草铵膦药液进行筛选,并对筛选到的植株进行PCR、RT-PCR检测。[结果]试验共得到19株草铵膦抗性植株;经检测,19株均为Bar阳性植物,其中5株含YB2目的基因,初步证明外源目的基因已整合到玉米基因组中。[结论]该研究为获得抗旱性好、产量高、安全的转基因玉米株系奠定了基础。[ Objective/ The aim was to introduce Yb＇2 gene into maize inbred hnes. [ Method J lanear expressing cassette commnmg YB2 gene was transferred into maize inbred line Jing 24 by pollen tube pathway method. To generation plants were screened by spraying 200 mg/L glufosi- nate herbicide,and the screened plants were detected by PCR and RT-PCR. ~ Result] 19 glufosinate-tolerant plants were obtained. All of them were Bar positive plants ,5 of them contained YB2 gene ,which proved that exogenous gene had been integrated into the genome of maize. [ Con- clusion ] The study laid a foundation for obtaining transgenic maize lines with good drought resistance, high yield and good security.

关 键 词：花粉管通道法 YB2基因 转基因玉米 

分 类 号：S188[农业科学—农业基础科学]