孟加拉进口黄鳝中致病性嗜水气单胞菌的分离与鉴定  

Isolation and Identification of Pathogenic Aeromonas hydrophila from Bangladesh Imported Eels

作　　者：王昱[1,2] 袁增壮 聂福平[1] 肖进文[1] 杨俊[1] 李应国[1] 叶自霞 李正国[2] 

机构地区：[1]重庆大学,重庆400044 [2]重庆出入境检验检疫局,重庆400020 [3]西南大学,重庆400716

出　　处：《安徽农业科学》2013年第24期9997-9999,10026,共4页Journal of Anhui Agricultural Sciences

基　　金：重庆市食品工程中心项目(2009CB10122);国家质检总局项目(201310093;2012IK029)

摘　　要：[目的]了解致病性睹水气单胞菌的存在状况和风险水平.[方法]采用分离培养、生化鉴定、序列测定和动物试验等方法,对2批孟加拉进口黄鳝进行致病性睹水气单胞菌检测.[结果]从孟加拉进口黄鳝体内分离出4株高度致病性的嗜水气单胞菌.嗜水气单胞菌对孟加拉黄鳝本身致病性不明显,但是对小鼠的致病性却高达10 000～ 100 000 CFU/ml.[结论]孟加拉进口黄鳝存在致病性睹水气单胞菌的感染,且对我国的食品和卫生安全存在一定的风险.[ Objective ] The research aimed to understand the status and risk level of pathogenic Aeromonas hydrophila. [ Method] By the meth- ods of isolation and culture, biochemical identification, sequencing and animal testing, pathogenic A. hydrophila in two batches of Bangladesh imported eels was detected. [ Result] Four highly pathogenic A. hydrophila were isolated and identified from two batches of Bangladesh eels. The pathogenicity of A. hydrophila to Bangladesh eel itself was not obvious and its pathogenicity to mice reached 10000 - 100000 CFU/ml. [ Conclu- sion] Bangladesh imported eel was infected with A. hydrophila and it had certain risk for China＇s food and health safety.

关 键 词：孟加拉黄鳝 致病性嗜水气单胞菌 分离 鉴定 

分 类 号：S965.131[农业科学—水产养殖]