应用Mab-basedAC-ELISA法对鸡IBDV分离株的抗原特性分析  

Antigenic characteristic analysis of chicken IBDV isolates with Mab-based AC ELISA

作　　者：张艳英[1] 高桂生[1] 贾青辉[1] 高光平[1] 李蕴玉[1] 张香斋[1] 孙继国[3] 张曼夫[2] 常火炬 李佩国[1] 

机构地区：[1]河北科技师范学院,河北预防兽医学重点实验室,河北昌黎066600 [2]中国农业大学,北京100193 [3]河北农业大学,河北保定071001 [4]邯郸市丛台区动物卫生监督局,河北邯郸056004

出　　处：《中国兽医学报》2013年第12期1828-1831,1837,共5页Chinese Journal of Veterinary Science

基　　金：河北省自然科学基金资助项目(300115);河北省科技攻关计划(12220408D);国家自然科学基金资助项目(31072004);河北科技师范学院科研创新团队(CXTD2012-01)

摘　　要：为了解传染性法氏囊病病毒(IBDV)的抗原变异分子基础,采用Mab-based AC-ELISA方法,利用11株单抗对2株河北IBDV分离株进行了抗原特性分析,并比较了超强毒株和弱毒株的VP2高变区序列的分子特征。结果显示:强弱毒株的抗原位点结合单抗的能力明显不同,除了Mab3、Mab9外,其余9个单抗的结合能力,超强毒株要高于弱毒株。超强毒株HeB-lf缺少了Mab3结合位点,弱毒株HeB-bdjz缺少了Mab3、Mab6结合位点,这说明强弱毒株不仅在序列上有差异,而且在抗原位点的缺失上也存在差异。本试验为控制IBD和疫苗改进具有重要意义。In order to understand molecular basis of antigenic variation on infectious bursal disease virus,antigenic characterstics of 2 IBDV isolates from Hebei province were analyzed to bind 11 Mabs with monoclonal antibody-base antigen capture ELISA（Mab-based AC-ELISA） in the test. And molecular characteristics of VP2 hypervariable regions were compared between very virulent isolates and attenuated isolates. The results showed that it is obviously distinct on ability of anti- gen site binding Mab. Apart from Mab3 and Mab9,in antigen capture ability of other 9 Mabs very virulent IBDV（vvIBDV）isolates are higher than that of attenuated isolates. Mab3 antigen-bind site was not detected in Hebei-lf isolates,while Mab3 and Mab6 site were both detected in HeB-bdjz i- solates. This suggested that it is different in the sequence and antigen site missing between very virulent IBDV and attenuated isolates.

关 键 词：鸡 IBDV Mab—based AC ELISA 抗原特性分析 

分 类 号：S852.65[农业科学—基础兽医学]