白牦牛卵泡液蛋白质组2-DE图谱的构建  

The construction of 2-DE map of white yaks follicular fluid proteome

作　　者：赵国顺[1] 陶金忠[2] 张勇[1] 王朝凤[1] 马艳粉[1] 李淑芳[1] 赵兴绪[1] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]宁夏大学农学院,宁夏银川750021

出　　处：《中国兽医学报》2013年第12期1885-1888,1895,共5页Chinese Journal of Veterinary Science

基　　金："十二五"农村领域国家科技计划(2013AA102505-3);国家自然科学基金资助项目(31001095)

摘　　要：分别用10%、12%、14%的SDS-PAGE胶分离蛋白质,比较不同浓度胶对白牦牛卵泡液蛋白双向电泳分离效果的影响;分别用丙酮沉淀法、热的SDS法和直接溶解法制备样品,比较不同制备方法对白牦牛卵泡液蛋白双向电泳分离效果的影响。结果表明,不同浓度胶对蛋白分离效果影响较大;丙酮沉淀法、热的SDS法和直接溶解法获得的蛋白点数分别为364±36、290±19和374±30个。对于白牦牛卵泡液蛋白来说,将其直接溶解后,用12%的胶可以得到较理想的2-DE图谱。To study the effect of different concentration homogeneous gels of SDS-PAGE on white yaks follicular fluid proteins of separation in two-dimensional gel electrophoresis, the gel concen- trations of 10%,12% and 14% were applied to separate follicular fluid sample. To study the effect of different sample preparation methods on white yaks follicular fluid proteins of separation in two-dimensional gel electrophoresis, the acetone precipitation, hot SDS methods and direct solubili- zation were applied to prepare follicular fluid sample. The result showed that there was a great difference in proteins of separation in different concentrations gels. The total protein number of samples processed by acetone precipitation,hot SDS methods and direct solubilization was 364± 36,290±19 and 374±30 respectively. For the separation of white yak follicular fluid proteins in 2- DE,direct solubilization and 12.5% homogeneous gel can obtain perfect maps of 2-DE.

关 键 词：白牦牛卵泡液 SDS—PAGE胶浓度 样品处理 双向电泳 

分 类 号：S814.1[农业科学—畜牧学]