圈养大熊猫食性转换前期肠道细菌多样性  

The diversity of intestinal bacterial flora of captive giant panda in the prophase of diet conversion

作　　者：罗永久[1] 何廷美[1,2] 钟志军[1] 王承东[2] 王晓艳[1] 吴虹林[2] 程安春[1] 何永果[2] 李才武[2] 张和民[2] 李德生[2] 杨海迪[2] 彭广能[1] 

机构地区：[1]四川农业大学动物医学院动物疫病与人类健康四川省重点实验室,四川雅安625014 [2]中国林业科学研究院大熊猫研究中心四川雅安碧峰峡基地,四川雅安625007

出　　处：《中国兽医学报》2013年第12期1896-1901,1906,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31272620);大熊猫国际合作资金项目(SD0632);四川省2011年人才培养资金;教育部"长江学者和创新团队发展规划"创新团队项目(IRT0848)

摘　　要：为了探讨大熊猫食性转换前期肠道菌群的多样性与差异性。对3只处于夏季食性转换前期大熊猫粪便细菌总DNA进行ERIC-PCR分析,并构建16SrDNA克隆文库,采用限制性内切酶HinfⅠ、MspⅠ对其进行RFLP(restriction fragment length polymorphism)及测序分析。研究表明,食性转换前期大熊猫肠道细菌主要由硬壁菌门和变形菌门组成。其中7月份样品中以梭菌属和醋杆菌属为主;8月份以八迭球菌属和埃希氏菌属为主;9月份以链球菌属和埃希氏菌属为主。此外在3个月中均发现一定比例的未培养细菌。结果表明:随着食性转换前期的过渡,大熊猫肠道菌群发生了改变。由硬壁菌门中的梭菌属渐变到以球菌属为主,由变形菌门的醋杆菌属渐变到以肠杆菌为主。To investigate the diversity and difference of giant pandas intestinal bacterial flora in the prophase of diet conversion. Total DNA of fecal bacterial was extracted from three giant pandas faecal samples in the period of summer diet conversion and analyzed by ERIC-PCR,and then 16S rDNA clone library was constructed and digested by Hinf I and Msp I ,at last the clone was se- quenced. Results showed that the giant pandas intestinal bacterial flora were mainly composed of Firmicutes and Proteobacteria in the period of diet conversion; Among them,samples in July con- sisted of Clostridiurn and Acetobacter;the main bacteria in August were Sarcinae and Escherichial In September the main bacterial were Streptococcus and Escherichia. In addition,a certain propor- tion of uncultured bacteria in the three months were found. The results indicated that with the transition of diet conversion, bacterial flora in giant panda gut changed. The main bacterial flora were Coccus in the Firmacutes and Enterobacteria other than Clostridium or Acetobacter in the Proteobacteria.

关 键 词：圈养大熊猫 食性转换前期 肠道菌群 

分 类 号：Q954[生物学—动物学]