镉对体外培养大鼠肝细胞自噬的影响  

Effects of cadmium on autophagy in primary rat hepatocytes cultured in vitro

作　　者：朱家桥[1] 王玲玲[1] 程来洋 王棋文[1] 王亨[1] 刘学忠[1] 刘宗平[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009

出　　处：《中国兽医学报》2013年第12期1907-1911,共5页Chinese Journal of Veterinary Science

基　　金：江苏省自然科学基金资助项目(BK2012264);江苏高校优势学科建设工程资助项目;扬州大学创新基金资助项目(027389604035349)

摘　　要：选用体外培养原代大鼠肝细胞为模型,醋酸镉处理之后,用Western blot和免疫荧光法检测了自噬标记分子LC3的表达水平,在此基础上,利用自噬激活剂(雷帕霉素)诱导自噬水平的升高,用MTT法分析了镉对细胞存活率的影响。结果显示,原代大鼠肝细胞在体外培养过程中自噬水平具有缓慢上升的趋势,而镉处理延缓了这种趋势,降低了自噬的水平和细胞存活率;利用激活剂增强自噬后不能减弱镉的损伤,表明镉有可能通过抑制自噬的保护作用,造成对肝细胞的损伤。In the present research,cadmium treated primary rat hepatocytes cultrued in vitro as the model. As a marker of autophagy,microtubule-associated proteinl light chain 3 （LC3） was exam- ined by western blot and immunofluorescence staining. Effect of cadmium on cell viability was ex- amined by MTT method, after rapamycin induce authophagy. The results showed that autophagy slowly increased during in vitro culture of primary rat hepatocytes, and cadmium treatment inhibi- ted this process by decreased autophagy and cell viability. However,activation of autophagy by ra- pamycin did not repair the damage of cadmium. These results indicate that cadmium may inhibit the protection of autophagy to damage the hepatocytes.

关 键 词：镉 自噬 大鼠 原代肝细胞 

分 类 号：S856.9[农业科学—临床兽医学]