苦马豆素抗血清治疗家兔小花棘豆中毒  

Treatment effects of serum against swainsonine on rabbits poisoned by Oxytropis glabra DC

作　　者：王帅[1] 陈根元[1] 张玲[1] 胡建军[1] 马春晖[1] 

机构地区：[1]塔里木大学动物科学学院新疆生产建设兵团塔里木畜牧科技重点实验室,新疆阿拉尔843300

出　　处：《中国兽医学报》2013年第12期1912-1916,1922,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31160497);新疆生产建设兵团博士基金专项(2008JC07);新疆生产建设兵团塔里木畜牧科技重点实验室开放课题(HS201207)

摘　　要：通过检测苦马豆素(swainsonine,SW)抗血清治疗小花棘豆中毒家兔血清免疫和生化指标变化,评价SW抗血清对家兔小花棘豆中毒的治疗效果。将18只家兔随机分为对照组(6只)、攻毒组(6只)和攻毒治疗组(6只),攻毒试验兔按干重拌料饲喂10g/(kg·d)小花棘豆草粉,攻毒组试验兔出现死亡时(第70天)停止攻毒。攻毒第21天,攻毒治疗组试验兔注射SW抗血清,每只兔每天0.5mL,连续注射4d。以攻毒试验开始进行首次采血,试验开始后每7d采血1次,进行血清免疫和生化指标测定。结果显示,攻毒第7天时攻毒家兔血清AKP活性和SW含量显著高于对照家兔(P<0.05);第14天时攻毒家兔血清AST和LDH活性显著高于对照家兔(P<0.05),血清AMA活性和E-玫瑰花环率均显著低于对照家兔(P<0.05);第21天时攻毒家兔血清BUN、CRE和GLU含量显著高于对照家兔(P<0.05)。攻毒治疗组家兔注射SW抗血清后血清AKP、LDH、AST、ALT活性及BUN、CRE、GLU、SW含量较攻毒家兔显著下降,AMA活性和E-玫瑰花环率显著上升。结果表明,SW抗血清可有效治疗家兔小花棘豆中毒。The serological and immunological index was detected to evaluate the effects of serum a- gainst SW on rabbits poisoned by Oxytropis glabra DC. Eighteen rabbits were randomly separated into control group （6 rabbits）,poisoning group （6 rabbits） and poisoning and curing group （6 rab- bits）. Poisoning rabbits were fed 10 g/（kg · d） power of Oxytropis glabra DC until rabbit in poi- soning grohp was dead. On d 21 after poisoning,rabbits in poisoning and curing group were treated by injecting 0.5 mL serum against SW for four days. Blood samples were collected before the poi- soning, and then once every 7 days for indexes of serological and immunological evaluation. The re- sult showed that the change of serum AKP and content of SW in poisoning groups were significant higher than that in control group on d 7（P〈0. 05） ,the change of serum LDH and AST in poison- ing groups were significant higher than that in control group on d 14（P〈0.05） ,the change of ser- um AMA and E-rosette in poisoning groups were significant lower than that in control group on d 14（P〈0. 05）, the change of serum BUN , CRE and GLU in poisoning groups were significant higher than that in control group on d 21（P〈0.05）. By injecting serum against swainsonine,the change of serum AKP, LDH, AST, ALT and content of BUN, CRE, GLU, SW in poisoning and cu- ring group was significant lower than poisoning group, the change of serum AMA and E-rosettein poisoning and curing group was significant higher than poisoning group. The results indicated that serum against SW had an offective of preventing Oxytropis glabra DC.

关 键 词：小花棘豆 苦马豆素抗血清 治疗 家兔 

分 类 号：S852.4[农业科学—基础兽医学]