石榴籽油调节2型糖尿病小鼠糖脂质代谢的药理研究  

Pharmacodynamics Study of Pomegranate Seed Oil in Regulating Type 2 Diabetic Mice Glycometabolism and Lipid Metabolism

作　　者：李艳[1] 荣向路[1] 高英[1] 辛永涛[1] 李卫民[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006

出　　处：《中药新药与临床药理》2013年第6期533-537,共5页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的观察石榴籽油(PSO)对2型糖尿病小鼠糖、脂质代谢的影响,探讨石榴籽油降糖、降脂的作用机制。方法将40只雄性C57BL/6N小鼠按照体质量、血脂水平随机分为正常对照组、模型组、二甲双胍组、石榴籽油组,每组10只。除正常对照组外,其余各组高脂饲料喂养,6周末,测定小鼠血糖、血脂水平;8周末,用高脂饲料结合腹腔注射链脲佐菌素(STZ)复制2型糖尿病小鼠模型;9周末,测定小鼠血糖、血脂、尿酸、总胆红素水平,以及观察肝脏载脂蛋白B100(Apo-B100)活性的变化。结果与模型组比较,石榴籽油组小鼠空腹血糖(FPG)、血浆总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)显著降低(P<0.05或P<0.01),尿酸、总胆红素水平也明显降低(P<0.05),肝脏Apo-B100含量明显降低(P<0.01),且石榴籽油降低TC的作用明显优于二甲双胍(P<0.01)。结论石榴籽油能降低糖尿病小鼠FPG,有效改善糖尿病小鼠的脂质代谢和尿酸代谢紊乱,且使肝脏Apo-B100水平维持正常,提高了机体血脂转运及代谢的能力,提示石榴籽油降糖、降脂效果可能与Apo-B100分泌减少有关。Objective To observe the effect of pomegranate seed oil（PSO） on glycometabolism and lipid metabolism and to explore the mechanism of PSO in decreasing blood glucose and lipid. Methods Forty male C57BL/6N mice were equally randomized into four groups based on the body weight and blood lipid levels： the normal control group, model control group, metformin group and PSO group. High fat food was given to the mice for 6 weeks, and then we tested the fasting plasma glucose（FPG）, total cholesterol（TC ） and triglyceride（TG） levels in the blood. At the end of week 8, the type 2 diabetic mice model was established by feeding high fat food combined with intraperitoneal injection of streptozotocin（STZ）. At the end of week 9, the blood glucose, blood lipid,uric acid（UA）, total bilirubin （TBil） levels and activity of hepatic Apo-B100 were measured. Results After treatment, FPG, TC, TG, low- density lipoprotein cholesterol（LDL-C） were decreased in PSO group, the differences being statistically significant（P 〈 0.05 or P〈 0.01 compared with the model group）. The uric acid and total bilirubin levels were also decreased, and Apo-B100 activity was improved in PSO group（P〈 0.05, compared with those in the model group）. PSO had better effect on decreasing TC than metformin （P〈 0.01 ）. Conclusion Pomegranate seed oil has an effect on reducing FBG and improving the disorder of lipid metabolism and uric acid metabolism in diabetic mice, and could maintain the hepatic Apo-B100 at the normal level, thus improving the ability of the body lipid transport and metabolism. It isindicated that the hypoglycemic and hypolipidemic mechanism of PSO may be associated with the decrease of Apo-B 100 level.

关 键 词：石榴籽油 血糖 血脂 载脂蛋白B100 

分 类 号：R285.5[医药卫生—中药学]