过山枫有效成分南蛇藤素和扁蒴藤素对斑马鱼节间血管生成的抑制作用研究  

Suppression Efect of Bioactive Celastrol and Pristimerin from Celastrus aculeatus Merr.on Zebrafish Intersegmental Vascular Development

作　　者：赵洋[1] 颜妙虹 白殊同[1] 佟丽[1] 陈晓辉[2] 

机构地区：[1]南方医科大学中医药学院,广东广州510515 [2]南方医科大学基础医学院,广东广州510515

出　　处：《中药新药与临床药理》2013年第6期537-540,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：美国国立卫生研究院(NIH)补充替代医学研究基金(FO5AT002013-03);广东省科技厅社会发展计划项目(2006B35604001);广州市科技局项目(2007JI-COO81)

摘　　要：目的观察过山枫有效成分南蛇藤素和扁蒴藤素对Fli1-GFP转基因荧光斑马鱼节间血管生成的影响。方法采用Fli1-GFP转基因荧光斑马鱼胚胎,胚胎发育至6 h后,分别加入不同浓度的南蛇藤素及扁蒴藤素与斑马鱼胚胎共同孵育,待胚胎发育至30 h时,荧光显微镜下观察并计数斑马鱼胚胎节间血管的数量、血管芽数,测量血管长度并计算新生血管长度抑制率。结果与阴性对照组比较,南蛇藤素与扁蒴藤素干预后,两组斑马鱼胚胎新生血管数量明显减少(P<0.01),血管芽增多(P<0.01),新生血管长度抑制率升高(P<0.01)。结论南蛇藤素及扁蒴藤素对斑马鱼节间血管生成有显著的抑制作用,这种作用与给药浓度呈正相关,南蛇藤素与扁蒴藤素比较,具有相近的新生血管抑制作用。Objective To investigate the anti-angiogenic effect of Celastrol and Pristimerin from Cel^Lstrus actdeattts Merr. Methods We observed and compared the number of intersegmental vessels （ISV） and vessel sprouts of the zebrafish under fluorescence microscope, and then counted the vessel length and inhibition rate after the F｜il-GFP transgenic zebra_fish embryo was treated with Celastrol and Pristimerin at different concentrations from fertilization hour 6 to 30 h. Results Compared with the negative control group, the number of [SV decreased markedly, and the number of vessel sprouts and inhibition rate were increased in the Celastrol group and Pristimerin group （P 〈 0.01 ）. Conclusion Celastrol and Pristimerin from Celastrus aculeatus Merr. have inhibitory effect on intersegmental vascular development in concentration-dependent manner, and Celastrol and Pristimerin have similar anti-angiogenesis effects.

关 键 词：过山枫 南蛇藤素 扁蒴藤素 血管新生 斑马鱼 

分 类 号：R285.5[医药卫生—中药学]