HPLC法测定骨康口服液中淫羊藿苷的含量  

Determination of Icariin in Gukang Oral Liquid by HPLC

作　　者：涂兴明[1] 李赐恩[2] 吴康郁[1] 

机构地区：[1]广州中医药大学附属骨伤科医院药剂科,广东广州510240 [2]广州中医药大学第一附属医院药剂科,广东广州510405

出　　处：《中药新药与临床药理》2013年第6期613-615,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省科技计划项目(2011B031700049)

摘　　要：目的建立HPLC法测定骨康口服液中淫羊藿苷的含量。方法采用HPLC法测定淫羊藿苷的含量，DiamonsilC18（4．6mmX250mm，5汕m）为色谱柱，流动相为乙腈-水（30：70），流速1．0mL·min-1，检测波长270nm。结果淫羊藿苷在0．0521-0．2084mg·mL-1范围内呈良好的线性关系（r=1．0000），回收率为99．61％（RSD=0．81％，n=9）。结论本法简便、准确、灵敏度高，重现性好，可用于骨康口服液中淫羊藿有效成分淫羊藿苷的含量测定。Objective To establish a quantitative method for icariin in Gukang oral liquid by HPLC. Methods HPLC was performed on Diamonsil C18 （4.6 mm×250mm, 5 μm）. The mobile phase was acetonitrile-water （30 ： 70） at a flow rate of 1.0 mL·min-1, the UV detection wavelength was 270 nm. Results The linear range of icariin was in the range of 0.0521 -43.2084 mg·mL-1 （r=l.0000）. The average recovery of icariin was 99.61% with RSD being 0.81%（n= 9）. Conclusion The method is simple, efficient and accurate, and can be used for the quality control of ieariin in Gukang oral liquid.

关 键 词：骨康口服液 淫羊藿苷 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]