HPLC-ELSD测定更年调和颗粒中黄芪甲苷的含量  

Determination of Astragaloside IV in Gengnian Tiaohe Granula by HPLC-ELSD

作　　者：张乐林[1] 周倩[1] 吴丽丽[1] 孙立立[1] 

机构地区：[1]山东省中医药研究院,山东济南250014

出　　处：《中药新药与临床药理》2013年第6期619-621,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：山东省科技发展计划项目(2008GG30002056);2010-2011年中医药行业科研专项(20110700705);山东省科技发展计划项目(2011-170);国家中医药管理局全国名老中医药专家传承工作室建设项目

摘　　要：目的建立HPLC—ELSD法测定更年调和颗粒q-黄芪甲苷的含量。方法色谱柱为ThermoSyncronisC18（250mmx4．6mm，5μm）；流动相为乙腈-水（34：66）；流速：1．0mL·min-1；柱温：30℃。蒸发光散射检测（ELSD）参数：漂移管温度85℃，氮气体积流量1．8mL·min-1。结果黄芪甲苷在0．812～8．12μg范围内具有良好的线性关系，加样回收率为98．07％（RSD为1．21％，n=6）。结论本法简单易行，重复性符合要求，测定结果准确，可作为更年调和颗粒中黄芪甲苷的含量测定方法。Objective To establish a HPLC-ELSD method for the determination of astragaloside IV in Gengnian Tiaohe Granula. Methods The HPLC-ELSD system consisted of Thermo Syncronis C18 column（250 mm×4.6mm, 5 μm） with the mobile phase of acetonitrile-water （34 ： 66）. The flow rate was 1.0 mL ·min-1, and the column temperature was set at 30℃. Evaporative light-scattering detector was adopted. The drift tube temperature of ELSD was set at 85℃, nitrogen volume flow rate was 1.8 L· min-1. Result Astragaloside IV showed a good linearity in the range of 0.812-8.12 μg. The recovery was 98.07 %（RSD=I.21%, n=6）. Conclusion The established method is simple and reliable, and is suitable for the determination of astragaloside IV in Gengnian Tiaohe Granula.

关 键 词：HPLC—ELSD 更年调和颗粒 黄芪甲苷 含量测定 

分 类 号：R284.1[医药卫生—中药学]