银杏叶中萜类内酯的稳定性研究  

Study on Stability of Terpene Lactones from Ginkgo Leaf

作　　者：林春颖[1] 梁莉君[2] 曾翠梅[1] 

机构地区：[1]广州中医药大学第三附属医院芳村分院,广东广州510360 [2]中山大学孙逸仙纪念医院,广东广州510120

出　　处：《中药新药与临床药理》2013年第6期622-624,共3页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的以银杏叶中萜类内酯化合物的含量为指标，评估其在不同环境下的稳定性。方法将银杏叶在酸、碱、氧化、加热及光照的加速降解条件下放置后，采用HPLC法测定银杏叶中银杏内酯A、B、C和白果内酯共4种萜类内酯的含量。色谱条件：费罗门C18分析柱（4．6mm×150mm，5μm）；甲醇一水（30：70）为流动相；漂移管温度：40℃；氮气压强3．4bar。结果银杏内酯A、B、C和白果内酯分别在0．995-24．875μg,0．568～14．200μg、0．489～12．225μg、0．495～12．375μg范围内线性关系良好，在酸碱条件，特别是酸性条件下出现降解现象。结论银杏叶中的萜类内酯在酸及氧化条件下稳定性稍差，储藏时需注意。Objective To investigate the stability of terpene lactones from ginkgo leaf. Methods Ginkgo leaf was exposed to acids, alkalis, oxidation, heating and light for accelerating the degradation. And then the contents of terpene lactones of ginkgolide A, ginkgolide B, ginkgolide C and blobalide in the compounds were determined by HPLC. The chromatographic conditions were as follows： Diamonsil C18 column （4.6 mm×150 mm, 5μm） , mobile phase consisting of Me0H-H20 （30 ： 70）, the temperature of drift tube being 40℃ and the nebulizer nitrogen pressure at 3.4 bar. Results The reliable liner range of ginkgolide A, ginkgolide B, ginkgolide C and blobalide was 0.995-24.875 μg, 0.568-14.200 μg, 0.489-12.225μg, 0.495-12.375μg, respectively. The degradation of ginkgo leaf was obvious especially under acidic condition. Conclusion Terpene lactones from ginkgo leaf show instability in acidic and oxidation conditions, which should be noted during storage.

关 键 词：银杏内酯A 银杏内酯B 银杏内酯C 白果内酯 稳定性 

分 类 号：R284.1[医药卫生—中药学]