离子交换树脂纯化黄连生物碱的工艺研究  

Purification of Total Alkaloid from Rhizoma Coptidis by Ion Exchange Resin

作　　者：魏金津[1] 姚曦[1] 何伟[1] 

机构地区：[1]广东药学院药物研究所/广东省药物新剂型重点实验室,广东广州510006

出　　处：《中药新药与临床药理》2013年第6期625-628,638,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：十一五国家科技重大专项(2009ZX09103-407)

摘　　要：目的优选离子交换树脂纯化黄连生物碱的工艺条件。方法以表小檗碱、黄连碱、巴马汀、小檗碱含量为指标,考察D113型、D001型、D151型、110型、732型阳离子交换树脂纯化黄连生物碱的效果及优选最佳树脂纯化工艺条件。结果 D151型离子交换树脂比其他树脂具有较优的吸附及洗脱参数;最佳纯化条件为:样品溶液浓度(以生药计)为0.1 g·mL-1、pH=9,流速为3 Bv/h,洗脱剂为40%乙醇酸性溶液(含10%醋酸),用量7 Bv,洗脱温度35℃。结论黄连提取液过D151型离子交换树脂柱前后各成分转移率均在95%以上,纯化效果好,适用于黄连生物碱的精制纯化。Objective To investigate the purification technology for total alkaloid from Rhizoma Coptidis by exchange resin. Methods With the contents of epiberberine, eoptisine, palmatine, and berberine as the indieat ion ors the effect of Dl13, D001, D151, 110 and 732 ion exchange resins in purifying the total alkaloids from Rhizoma Coptidis was evaluated, and the ion exchange resin purification condition was optimized. Results It has been found that D151 resin had the best adsorption and elution capacity for total alkaloids. The optimal process was as follows： the concentration of the sample solution was 0.1 g·mL-1 and pH value was 9 with flow rate at 3 BV/h, eluting at 35 ℃ with 40 % ethyl alcohol acid solution（with 10 % acetic acid） 7 times as much as the resin volume. Conclusion Under the optimized conditions, the proportion of total alkaloid is over 95 %, showing excellent purification of total alkaloid from Rhizoma Coptidis.

关 键 词：黄连 离子交换树脂 总生物碱 精制工艺 

分 类 号：R284.1[医药卫生—中药学]