养血消风饮对慢性变应性接触性皮炎小鼠Th1/Th2免疫平衡失调的影响  

Experimental Study on Influence of Yangxue Xiaofeng Drink on Th1/Th2 Immune Imbalance of Chronic ACD Mice Model

作　　者：芦源[1] 黄瑾[1] 关洪全[2] 

机构地区：[1]辽宁中医药大学附属医院皮肤科,辽宁沈阳110032 [2]辽宁中医药大学基础医学院,辽宁沈阳110847

出　　处：《辽宁中医药大学学报》2013年第12期45-47,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：辽宁省教育厅基金资助项目(2009A502)

摘　　要：目的 :利用小鼠耳廓ACD模型进行养血消风饮的药效学研究,从Th1/Th2网络平衡的角度进行其免疫学机制探讨,为研究药物的作用机理提供依据。方法 :用二硝基氯苯(DNCB)建立小鼠耳廓慢性ACD模型,随机分为4组:空白组、模型组、中药组、氯雷他定组,对比观察小鼠血清中IFN-γ和IL-4含量,以及小鼠病变耳组织中IFN-γmRNA及IL-4mRNA的表达情况,并进行相关性分析。结果 :养血消风饮减少模型小鼠血清中IFN-γ的含量和增加IL-4含量,并能促使小鼠病变耳组织中IFN-γmRNA低表达和IL-4mRNA的高表达。结论 :养血消风饮可能在转录水平和分子水平对ACD小鼠的Th1/Th2网络平衡失调起到调节作用。Objective ： To test the efficiency of the herbal medicine Yangxue Xiaofeng Drink by using the mice models of ACD on its auricles. From the immune balance of Thl/Th2 to explore the immunological inhibition mechanism further in-depth. Methods： Chronic ACD mice model was built up by stimulating its auricles by DNCB, then all the mice were separated into four groups： blank model group, model group, traditional Chinese medicine treatment group, Western medicine control group. The levels of IFN-γ and IL-4 in the serum of ACD mice model were measured by using ELISA method, using RT-PCR to detect lesions local IFN- γ/mRNA, IL-4 mRNA expression, and then the correlation of serum cytokine protein expression and mRNA expression in lesions were analyzed. Results ： Yangxue Xiaofeng Drink can raise level of IL-4 and reduce that of IFN- γ in serum of ACD model, while increase expression level of IL-4 mRNA and reduce that of IFN- γ mRNA in ear tissue. Conclusion ： Yangxue Xiaofeng Drink may adjust the network of Th 1/Th2 immune balance of chronic ACD mice model at the transcriptional level and molecular level.

关 键 词：养血消风饮 变应性接触性皮炎 TH1 Th2网络平衡 实验研究 

分 类 号：R758.22[医药卫生—皮肤病学与性病学]