安徽银莲花根茎中主要皂苷成分高效液相色谱法含量测定  

Content Determination of Hederasaponin B in Anemone Anhuiensis

作　　者：张丽萍[1] 谭璇[2] 侯安伟[1] 黄芳[1] 韩林涛[1] 

机构地区：[1]湖北中医药大学中药资源与中药复方教育部重点实验室,湖北武汉430065 [2]华中科技大学同济医学院附属协和医院胃肠外科,湖北武汉430022

出　　处：《辽宁中医药大学学报》2013年第12期81-82,共2页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(31000158;30870255)

摘　　要：目的：分析测定安徽银莲花中主要皂苷成分HederasaponinB的含量。方法：采用C18（AgilentZORBAXEclipseXDB-C18,4.6mm×150mm）分析柱,流动相为甲醇-水（55：45）,流速1.0mL/min,柱温30℃,检测波长210nm。进样量对照品溶液和供试品溶液各10μL。理论塔板数以HederasaponinB峰计不低于3000。结果：HederasaponinB在1.10~5.20μg范围内进样量与峰面积呈良好的线性关系（r=0.9996）,平均回收率为100.95%,RSD=1.04%（n=6）。HederasaponinB在不同5批次药材中评价含量为0.350%,RSD=1.94%。结论：该方法简便、灵敏、重复性好,可用于对安徽银莲花的品质评价,为鹅掌草近缘种质量控制及开发提供实验依据。Objective：To determine Hederasaponin B in Anhui anemone by HPLC. Methods： Hederasaponin B was separated on Agilent ZORBAX Eclipse XDB-C18 column （4.6 mm× 150 mm） with a mobile phase of methanol-water （ 55 ： 45 ）, detecting at a wavelength of 210 nm. The detection wavelength was set at 210 nm, and the column temperature was constantly kept at 40℃. The reference solution with sample solution injection volume was 10 pc L. On the plate number to Hederasaponin B, the peak count of was not less than 3000. Results ： The calibration curve was linear in the range of 1.10 μg-5.20μg （ r=0.9996 ）. The average recovery rate was 100.95% and RSD--1.04% （ n=6 ）. Hederasaponin B in different batches of five herbs evaluation content was 0.350%, RSD = 1.94%. Conclusion ： The method is simple, sensitive and precise. It can he used for the quality control of Anemone anhuiensis and provides experimental evidence for the quality evaluation and development of the related plants of Anemone tlaceida.

关 键 词：鹤峰银莲花 鹅掌草 HederasaponinB HPLC 

分 类 号：R284.2[医药卫生—中药学]