钙对化学诱抗剂诱导番茄叶片木质素合成的影响  

Effect of calcium on lignin synthesis induced by chemical elicitors

作　　者：余朝阁[1,2] 黄欣阳[2] 李天来[1] 刘志恒[2] 

机构地区：[1]沈阳农业大学园艺学院,设施园艺省部共建教育部重点实验室,沈阳110161 [2]沈阳农业大学植物保护学院,沈阳110161

出　　处：《植物营养与肥料学报》2013年第6期1445-1449,共5页Journal of Plant Nutrition and Fertilizers

基　　金：辽宁省教育厅项目(L2011115);国家"十二五"科技支撑计划项目(2011BAD12B03);辽宁省重大科技攻关项目(2011215003)资助

摘　　要：为探索钙对化学诱抗剂水杨酸(SA)、茉莉酸甲酯(MeJA)、龙胆酸(GA)和β-氨基丁酸(BABA)诱导番茄木质素合成的影响,在番茄六叶期用上述诱抗剂分别处理第3叶片,然后喷施CaCl2、蒸馏水、乙二醇-双-(2-氨基乙醚)四乙酸(EGTA)或LaCl3,处理后0、1、2、3、5 d测定各处理番茄第3叶片(诱导叶)和第5叶片(非诱导叶)中木质素含量的变化。结果表明:(1)用上述化学诱抗剂处理番茄第3叶片,诱导叶及其上位非诱导叶中木质素含量均升高;(2)外源Ca2+进一步促进上述诱抗剂诱导番茄叶片中木质素含量的增加;而Ca2+螯合剂EGTA和质膜钙通道抑制剂LaCl3,则不同程度地抑制这些诱抗剂对木质素含量的诱导作用。这些结果表明,钙对上述4种诱抗剂诱导番茄叶片中木质素的合成具有正调控作用。The effects of calcium on synthesis of lignin induced by chemical elicitors in tomato were investigated in this study.The 3rd leaves were smeared with chemical elicitors：salicylic acid（SA）, Methyl jasmonate（MeJA）,β-aminobutyric acid （ BABA ） and gentisic acid （ GA ） separately at the six-leaf stage of tomato .After the third leaves dried under room temperature , all the tomato plants were sprayed with CaCl 2 , distilled water , EGTA and LaCl3 in turn.The contents of lignin in the third leaves （ induced ） and the fifth leaves （ un-induced ） were determined in 0, 1, 2, 3 and 5 days after treatments.The results show that：（1） The lignin content in both the induced leaves and un-induced leaves increased after treated with chemical elicitors .（ 2 ） The lignin synthesis in treated tomato leaves were further promoted by exterior Ca 2＋, but inhibited by Ca 2＋ chelator EGTA and the plasmalemma Ca 2＋ channel blocker La 3 ＋ in different levels .These results indicated that Ca 2＋play a positive regulation role in synthesis of lignin in tomato leaves induced by the above four chemical elicitors .

关 键 词：番茄 钙 化学诱抗剂 木质素 

分 类 号：S641.2[农业科学—蔬菜学]