河南省烟草低头黑病的病原鉴定  

Identification of Pathogen Causing Tobacco Black Death Disease in Henan Province

作　　者：王海涛[1] 苗圃[2] 李淑君[1] 赵丹[2] 白静科 康业斌[2] 

机构地区：[1]河南省农业科学院烟草研究所,河南许昌461000 [2]河南科技大学林学院,河南洛阳471000

出　　处：《河南农业科学》2013年第11期86-89,共4页Journal of Henan Agricultural Sciences

基　　金：中国烟草总公司项目(110200902065);河南省烟草公司项目(2010)

摘　　要：为了明确引起河南省烟草低头黑病的病原菌,在襄城、舞阳两县采集表现烟草低头黑病症状的病株,采用组织分离与单孢分离相结合的方法分别获得Ⅰ号和Ⅱ号2个菌株。形态学鉴定结果表明:在PDA培养基上,菌落正面初期呈白色、黄色或灰色等变化,但后期均能产生分生孢子盘、刚毛、分生孢子梗与分生孢子。rDNA-ITS序列分析结果表明,菌株Ⅰ号和菌株Ⅱ号属于同一个种,其序列与序列号为DQ286158.1和HM231266.1的辣椒炭疽菌(Colletotrichum capsici)ITS序列同源性分别均为100%和99%。因此,河南省烟草低头黑病的病原菌可确定为辣椒炭疽菌[Colletotrichum capsici(Syd.)Butler&Bisby]。In order to identify the pathogen causing tobacco black death disease in Henan prov-ince ,two strains were isolated from the disease infected plants in Xiangcheng and Wuyang by the methods of tissue separation and single spore isolation .The pathogens were identified based on their morphological characteristics and ITS sequences .Studies on the pathogens showed that colo-nies were initially white ,yellow or gray on PDA medium ,and they all produced acervuli ,bristles , conidiophores ,and conidia .Two strains were identified as the same species based on their rDNA-ITS ,which displayed 100% and 99% identities with DQ286158 .1 and HM231266 .1 ,respectively . It is confirmed that Colletotrichum capsici（Syd .） Butler ＆ Bisby is the causal organism of tobac-co black death disease in Henan province .

关 键 词：烟草 低头黑病 辣椒炭疽菌 鉴定 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]