硫化氢下调MMP-9的表达抑制肝癌HepG2细胞的迁移  

Hydrogen sulfide downregulates MMP-9 expression and inhibits migration of HepG2 cells

作　　者：杨云[1] 董琳[2] 黄圣旺[3] 王义刚[1] 秦春宏[1] 

机构地区：[1]南华大学附属第二医院,湖南衡阳421001 [2]南华大学肿瘤研究所,湖南衡阳421001 [3]浙江省苍南县人民医院,浙江温州325800

出　　处：《中国药理学通报》2013年第12期1676-1679,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81172365);浙江省温州市卫生局资助项目(No 2013A17)

摘　　要：目的研究硫化氢(H2S)对人肝癌HepG2细胞迁移及MMP-9表达的影响。方法划痕愈合和Transwell实验检测H2S供体硫氢化钠(NaHS)对HepG2细胞迁移的影响;Western blot检测NaHS对HepG2细胞MMP-9表达的影响。结果划痕实验结果显示,200和400μmol·L-1的NaHS作用HepG2细胞16 h后,较未处理组划痕距离明显增加(P<0.05),表明H2S可抑制HepG2细胞的愈合能力。Transwell实验结果显示,100、200和400μmol·L-1的NaHS作用HepG2细胞24 h后,穿膜细胞数分别为(18.3±1.16)、(16.3±1.52)、(12.3±0.58)个,较未处理组的(24.0±1.73)个明显减少(P<0.05),表明H2S可抑制HepG2细胞迁移。Western blot结果显示,100、200和400μmol·L-1的NaHS作用HepG2细胞24 h后,MMP-9蛋白表达明显降低(P<0.05)。结论 H2S可抑制人肝癌HepG2细胞的迁移,其机制可能与下调MMP-9有关。Aim sulfide （ H2 S） on To investigate the effects of hydrogen the migration of human hepatoeellu- lar carcinoma HepG2 cells and its effects on MMP-9 expression in HepG2 cells. Methods The effects of a H2S donor sodium hydrosulfide （NariS） on HepG2 cell migration were examined by Wound Healing assay and Transwell assay, and the expression of MMP-9 in HepG2 cells was examined by Western blot. Results Wound healing assay discovered that the scuffing dis- tance of HepG2 cells exposed to 200 and 400 μmol·L^-1 NariS for 16 h was significantly increased com- pared with that of no-treatment group, indicating H2S could inhibit the migration in HepG2 cells （P 〈 0.05）. Transwell assay showed that the number of HepG2 cells migrated through the Matrigel treated by 100, 200 and 400 μmol·L^-1 NariS for 24 h was 18.3±1.16, 16.3± 1.52 and 12.3 ±0.58, respectively, which was obviously decreased compared with 24.0 ± 1.73 of non-treatment group （ P 〈 0. 05 ）, indicating HzS could inhibit the migration in HepG2 cells. West- ern blot showed that the expression of MMP-9 was down-regulated in HepG2 cells treated by 100, 200 and 400 μmol·L^-1 Naris for 24 h, respectively. West- ern blot demonstrated that the expression of MMP-9 protein was decreased. Conclusion H2S may inhibit the migration of human hepatocellular carcinoma HepG2 cells, which may be related to the down-regula- tion of MMP-9.

关 键 词：硫化氢 硫氢化钠 肝癌 HEPG2细胞 细胞迁移 MMP-9 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R394.2[医药卫生—基础医学]